Equipment
Technologies
Equipaments
Image analysis
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We have:
- Olympus BH2-UMA optical microscope connected to a Nikon D70 digital camera.
- Digital camera.
- Collection column.
- Workstations.
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Cytometry
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We have:
Bellvitge Campus:
- Beckman Coulter MoFlo Astrios separator cytometer, 4 lasers, 14 colors.
- Becton Dickinson FACSCanto II analyser cytometer, 2 lasers, 6 colors.
- FACScalibur analyser cytometer, 2 lasers, 4 colors.
- Beckman Coulter Gallios analyser cytometer, 3 lasers, 10 colours.
- Fluostar optima microplate reader (bmg) with double injection system and temperature regulator.
Diagonal Campus:
- Mass cytometer (Cytof) Helios (Fluidigm).
- Aria FUSION (Becton Dikinson) cell sorter, with flow hood, 4 lasers, 16 colors.
- Aria SORP (Becton Dikinson) cell sorter, 4 lasers, 18 colors.
- Gallios (Beckman Coulter) flow cytometer, 3 lasers, 10 colors.
- Aurora (Cytek) flow cytometer, 4 lasers, spectral.
- Magpix (Luminex)for soluble antigens detection.
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Electron cryomicroscopy
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We have:
- Spirit TWIN (FEI) 120 kV LaB6 Tecnai TEM cryomicroscope with Megaview 1kx1k CCD and tomograph.
- Tecnai G2 F20 (FEI) 200 kV FEG TEM cryomicroscope with Eagle 4kx4k CCD and tomograph.
- Jeol JEM 1010 100 kV TEM microscope with Megaview 1kx1k CCD.
- Leica DM2000LED fluorescence microscope.
- Leica UC6/FC6 cryoultramicrotome with cryosphere.
- Leica UCT ultramicrotome.
- Ultracut E ultramicrotome.
- Leica UC7/FC7 cryoultramicrotome.
- Vitrobot Mark III (FEI) immersion cryofixation/vitrification .
- Leica AFS cryosubstitution (2).
- Leica EM-AFS2 freeze substitution.
- Baltec 060 BAF cryofracture/freeze-etching.
- In situ laboratory: paraffin microtome, optical microscope and accessories.
- Leica EM HPM100 high-pressure freezing.
- Leica EM-ACE 600 high-vacuum carbon coater.
- Modular Stereomicroscope Leica MZ6 with SFL 100 (fluorescence module).
- Eppendorf 5804R refrigerated centrifuge.
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Animal Experimentation Facility
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We have:
- Cages and stable stalls.
- Glass aquaria and polyester tanks.
- Biological safety cabinets.
- Extractor hoods.
- Centrifuges.
- Experimental operating room.
- Surgical magnifying glasses.
- Ventilators for assisted breathing.
- Gas anaesthesia equipment.
- Perkin Elmer In vivo IVIS-Lumina III Bioluminescence and Fluorescence equipment.
- Hamamatsu Aequoria Bioluminescence and Fluorescence equipment.
- Rodent stabling under conventional conditions.
- Rodent stabling under SPF conditions.
- Rodent stabling under negative pressure conditions.
- Rabbit stabling under conventional conditions.
- Pig stabling under conventional conditions.
- Experimental labs in the conventional zone.
- Experimental labs in the SPF zone.
- Behavior labs in the conventional zone.
- Behavior labs in the SPF zone.
- Ventilated racks.
- Filter cages.
- Metabolic cages.
- Confocal Laser Scanning Microscope and Multiphoton with Resonant Scanner. With temperature control.
- 7T Experimental Magnetic Resonance imaging equipment.
- Biological irradiator.
- Software with tracking system SMART 3.0.
- Thermal blankets.
- Triple beam balance.
- Precision balance.
- Hydrogen peroxide SAS.
- UV SAS.
- Air shower.
- ISO 9001:2015 quality management system.
- AniBio, Unit management software.
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Genomics
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We have:
Bellvitge Campus:
- Applied Biosystems ABI Prism 7900 HT quantitative real-time PCR.
- Applied Biosystems QuantStudio 7 pro quantitative real-time PCR.
- Chemagen Magnetic nucleic acid isolation module.
- Agilent Technologies Bioanalyzer 2100.
- BMG Labtech Fluostar Optima Microplate reader with double injection system and temperature control.
Diagonal Campus:
- Applied Biosystems ABI Prism 3730 capillary electrophoresis DNA sequencer (48 capillary).
- Applied Biosystems ABI Prism 3730XL capillary electrophoresis DNA sequencer (96 capillary).
- Applied Biosystems ABI Prism 7900 HT quantitative real-time PCR.
- Roche Light Cycler 480 II quantitative real-time PCR.
- Applied Biosystems QuantStudio 7 pro quantitative real-time PCR.
- Agilent Technologies Bioanalyzer 2100.
- Applied Biosystems thermocyclers: PCR ABI 9700, PCR ABI 9800 Fast, PCR ABI 2400 and 2720; MGW Biotech PCR Primus thermocycler; Techne PCR TC-412 thermocycler.
- Beckman Coulter Biomeck NXP robotic workstation.
- Qiagen Mixer Mill MM 300 for grinding and homogenizing samples .
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Electronic microscopy (TEM/SEM)
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We have:
Transmission electron microscope (TEM):
- Transmission electron microscope JEOL 1010 + Orius (Gatan) CCD camera.
- Ultracut E ultramicrotome.
- Reichert-Jung Ultracut ultramicrotome.
- Leica UC7 ultramicrotome with digital IC90-E camera.
Scanning electron microscope (SEM):
- Scanning electron microscope JEOL JSM-7001F with secondary and retro-dispersed electron detectors.
- Polaron Critical Point Drying and and Emitech Critical Point Drying.
- Fisons Coating System SEM.
- Q150Tplus (Quorum) carbon evaporator.
Preparation laboratory:
- MIKRO 200R (Hettich) refrigerated centrifuge.
- 13/13 Dr Autocal (Roebling) osmometer.
- pH-2001 (Crison) pH-meter.
- OLYMPUS CX41RF optical microscope with OLYMPUS DP70 digital camera.
- MOTIC BA410E optical microscope with 3+ Moticam digital camera.
- SCZ-T4P Leica binocular magnifier.
- SMZ745T Nikon binocular magnifier.
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Advanced optical microscopy
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We have:
- Culture room.
- Fiji, Image J, MetaMorph, Imaris and Huygens image treatment workstations.
Diagonal Campus:
- Leica CM3050S cryostat
- Leica DMIRB wide-field transmitted light and fluorescence microscope.
- Leica MLZIII stereo fluorescence microscope.
- Leica RM 2135 microtome
- Leica SPII confocal microscope with spectral detection.
- Leica TCS-SPE spectral confocal microscope.
- Leica Thunder high-speed fluorescence microscope.
- Zeiss LSM880 high speed spectral confocal microscope.
- Ted-Pella XH-4002 paraffin dispenser.
Campus Clínic:
- Leica TCS-SP5 high speed, multiphoton, spectral confocal microscope.
- Leica DMI6000 B wide-field fluorescence microscope.
- Leica ASTP microinjector.
- Zeiss LSM880 high speed spectral confocal microscope.
Bellvitge Campus:
- Axio Imager M2 Apotome 3 fluorescence microscope.
- Carl Zeiss SteREO Lumar V12 stereo fluorescence microscope.
- Leica DMIRB Microscope.
- Leica TCS-SL confocal microscope with spectral detection.
- Nikon E800 Microscope.
- Zeiss LSM880 high speed spectral confocal microscope.
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Proteomics
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We have:
Electrophoresis:
- Amersham Biosciences IPGphor 2-D electrophoresis protein separation system and Bio-Rad PROTEAN-IEF
Image analysis:
- Amersham Biosciences DeCyder DIGE enabled TYPHOON TRIO fluorescent scanner.
Scission and digestion of gel stains:
- Genomic Solutions Investigator ProGest digestion unit (up to 96 samples).
Mass spectrometry and liquid chromatography:
- LTQ-Orbitrap Velos ion trap mass spectrometer with Thermo Finnigan electron transfer dissociation (ETD) accessory.
- Thermo Finnigan LCQ DECA XP ESI ion trap.
- Applied Biosystems MALDI-TOF Voyager DE perspective mass spectrometer.
- Proxeon Nano LC Easy and Eksigent Nano LC Ultra AS2 nano liquid chromatographs.
Data analysis:
- Licences for Protein Prospector, SEQUEST and Proteome Discoverer 1.2 from Thermo Finnigan , and PEAKS from BioInformatics Solutions.
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SPM nanobioscience techniques (AFM, STM)
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We have:
- Atomic force microscopes (AFM):
- Bruker Multimode 8 with Nanoscope V controller electronics.
- Bruker Dimension 3100 with Nanoscope IV controller electronics.
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Technical unit for radiological protection
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We have:
- Biological irradiator of animals and cells Shepherd Mark I30.
- Hidex model 600 SLe liquid scintillation counters.
- Wallac models 1209 and 1409 liquid scintillation counters.
- Packard models 1500, 2100, 2810 and 2900 liquid scintillation counters.
- Wallac Compugamma solid scintillation gamma counters.
- Packard Cobra II solid scintillation gamma counters.
- Biorad Molecular Imager FX and FX Pro Plus electronic autoradiography units.
- Molecular Dynamics Typhoon 8600 electronic autoradiography unit.
- FAG Contamat FHT-111M surface contamination measuring unit.
- Thermo Scientific (RadEye) NE Technology PDM1A radiation level measuring equipment.
- Atomtex AT1123 X-ray radiation level measuring equipment.
- RTI Electronics Black Piranha,ETR1,Sedentex mannequin, etc. quality control equipment for X-ray units.
- Hewlett Packard Faxitron 43855 A X-ray booth.
- Fuji Medical Film FPM 100A automatic radiographic film developer.
- Rad Elec Inc (Electets E-Perm) radon measurement system equipament in air.
- Specific equipment for the management of radioactive waste and radiological protection.
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Surface analysis (ESCA / Auger)
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We have:
- PHI ESCA-5500 photoelectron spectroscope.
- SPECS SAGE-HR photoelectron spectroscope .
- SAM-670 PHI Auger microscope.
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X-ray diffraction (XRD)
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We have:
- PANalytical X’Pert PRO MRD diffractometer; high-resolution texture goniometer; parallel and mono-capillary optics; Bartels monochromator; PIXcel detector .
- PANalytical X’Pert PRO MPD theta/theta diffractometer; reflection and transmission configurations; 45-position sample loader; Oxford Cryosystems 700 Cryostream series cryostat; PIXcel detector.
- PANalytical X’Pert PRO MPD alpha1 diffractometer; focusing primary monochromator; 15-position sample loader; X’Celerator detector.
- PANalytical X’Pert PRO MPD theta/theta diffractometer; Bragg-Bentano configuration; A PAAR HTK1200 high-temperature chamber; X’Celerator detector.
- INEL CPS-120 diffractometer; 120º curved position detector; primary monochromator and parabolic mirror; FURCAP INEL temperature chamber .
- Siemens D-500 diffractometers; grazing incidence and divergent optics; A PAAR TTK temperature chamber; scintillation detectors; Braun PSD position detector.
- X-ray single crystal diffractometer Bruker D8 Venture. Cu and Mo microbeam sources. 4 circles Kappa goniometer. Integrated CCD video microscope. CMOS area detector Photon 100. Bruker Kryoflex II cryostat (80 - 400 K)
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Granulometry
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We have:
- Beckman Coulter LS 13 320 laser-diffraction particle dimension analyser.
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Transmission electron microscope (TEM) applied to materials
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We have:
- JEOL JEM 2100 transmission electron microscope with accessories for EDX analysis, STEM annular dark field, precession and tomographic detectors.
- Philips CM30 300 kV transmission electron microscope.
- Hitachi 800MT electron microscope with Gatan multiscan chamber .
- JEOL JEM 2010F 200 kV transmission electron microscope with field emission tube with electron energy loss spectrometer (EELS) and image filter (GIF) and Z-STEM unit with high-angle annular detector.
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Scanning electron microscopy
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We have:
- Quanta 200 FEI, XTE 325/D8395 environmental scanning electron microscope.
- Jeol J-6510 scanning electron microscope (Freezing unit, GATAN ALTO-1000, EDS detector, backscattered electrons detector).
- JEDL J-7100 field emission scanning electron microscope (Cathodeluminiscence spectrometer GATAN MONO-CL4, EDS detector, retroscattered electron detector).
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Paleomagnetism
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We have:
- 2G Enterprises 2G SRM755R superconducting rock magnetometer.
- ASC IM10-30 impulse magnetizer.
- Geofyzica Brno KLY-2 magnetic susceptibilimeter.
- JR6A (Agico) spinner magnetometer.
- Magnetic Measurements Ltd. MMDT80 thermal demagnetizer.
- MFK2 Kappabridge with a CS4 temperature unit and a CS-L cryostat (AGICO).
- Schonstedt Instruments TSD1 thermal demagnetizer.
- Schonstedt Instruments GSD-5 alternating field demagnetizer.
- TD48EU (ASC Scientific) thermal demagnetizer.
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Mechanical, electronic, and vacuum technology
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We have:
- Goratu Group Lagun FU-TV 1250 and Lagun GVM 5-SP universal milling machines equipped with length displays on all three axes and precision accessories .
- Pinacho S-90/225 lathe equipped with display, able to work with diameters of 400 mm.
- Harrison M-300 lathe .
- Metal shears for cutting sheet metal .
- HB320SV drilling saw and saw for flat surfaces .
- Lincoln Electric Invertec V200-T welding equipment .
- Erlo-SPV drill with movable bench .
- Precision and measurement tools.
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Metal analysis (ICP-MS, ICP-OES, XRF, AA)
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We have:
- Agilent 7500ce ICP-MS spectrometer with reaction cell.
- Milestone DMA-80 direct mercury analyzer.
- Perkin-Elmer Nexion 350d ICP-MS spectrometer with reaction cell.
- Perkin-Elmer Elan-6000 ICP-MS spectrometer.
- Perkin Elmer Optima 3200 RL simultaneous ICP-OES spectrometer.
- Perkin Elmer Optima 8300 simultaneous ICP-OES spectrometer.
- ICP-MS multi-collector Nu Instruments Plasma 3 spectrometer.
- Panalytical, Axios PW 4400/40 sequential wavelength-dispersive X-ray spectrophotometer (WDXRF).
- Varian AA240FS atomic absorption spectrophotometer with flame.
- Multi-Collector Coupled Induction Plasma Mass Spectrometer (MC-ICPMS) Nu Instruments Plasma 3.
- Aridus II desolvator sampling system .
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Applied gas chromatography–mass spectrometry
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We have:
- QP2010 gas chromatograph coupled to mass spectrometer (Shimadzu)
- TRACE GC Ultra gas chromatograph coupled to ITQ 900 (250 lt)-Upgrade MSMS mass spectrometer (Thermo Fisher Scientific)
- TRACE gas chromatograph coupled to DSQII mass spectrometer (Thermo Fisher Scientific) with TRIPLUS automatic injector Head Space module
- FocusGC–DSQII gas chromatograph (Thermo Fisher Scientific) with UNITY Series II thermal desorption system(Markes International)
- 6890N gas chromatograph with a 5975 Inert XL mass spectrometer (Agilent Technologies)
- 7890A gas chromatograph with a 5975C Inert XL mass spectrometer (Agilent Technologies). Gerstel autosampler (injectors cis,TDU, HS, PTV)
- 5973 mass spectrometer with a direct introduction probe (Agilent Technologies)
- GC2025 gas chromatograph with a FID detector (Shimadzu)
- TRACE 1300 gas chromatograph with ECD and TCD detectors (ThermoFisher Scientific)
- Cryogenic mill SPEX Certiprep 6850
- Elemental analysers CE1108, Flash 2000 and Flash1112 (Thermo Scientific)
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Radiocarbon dating
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We have:
- Combustion tube under an oxygen stream.
- Combustion pump under pressurized oxygen.
- Chemical system for obtaining carbon dioxide by wet basis, acid decomposition or oxidation.
- Chemical system for purifying carbon dioxide.
- Chemical system for reducing carbon dioxide to benzene.
- LKB-Wallac Rackbeta 1217 liquid scintillation counter.
- Packard LL/TL 2255 liquid scintillation counter.
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Isotope-ratio mass spectrometry (IRMS)
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We have:
- MAT 253 isotope-ratio mass spectrometer (ThermoFisher Scientific) with:
- Kiel IV carbonate device
- Gas Bench system, Precon preconcentrator and Pal automatic sampler
- MAT 253 isotope-ratio mass spectrometer with Kiel III carbonate device
- Delta Plus XP isotope-ratio mass spectrometer (ThermoFisher Scientific) with:
- TC-EA pyrolizer with solid sampler
- TC-EA pyrolizer with liquid sampler
- Carlo Erba 1108 element analyzer
- MAT 253Plus isotope-ratio mass spectrometer (ThermoFisher Scientific) with:
- GC Trace 1310 gas chromatograph with Triplus RSH sampler and GC Isolink II and ConFloIV interfaces
- Gas Bench system, Precon preconcentrator and Pal automatic sampler
- Delta V Advantage isotope-ratio mass spectrometer with a Trace GC gas chromatograph and GCIsolink and ConFlo IV interfaces (ThermoFisher Scientific)
- Delta V Advantage isotope-ratio mass spectrometer with a Flash IRMS element analyzer and a CONFLO IV interface (ThermoFischer Scientific)
- Delta Plus XP (isotope-ratio mass spectrometer (ThermoFisher Scientific) with:
- Agilent 6890 gas chromatograph abd GC Combustion III interfaces
- Surveyor gas chromatograph and LC Isolink interfaces
- Picarro L2130i analyzer with a H2O stable isotopes HDS-2412 vaporizer
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Molecular spectroscopy
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We have:
- Analytik Jena Specord 205 UV-VIS spectrophotometer
- Thermo IN10MX Fourier transform infrared spectrometer with imaging microscope
- Perkin Elmer Frontier Fourier transform infrared spectrometer
- Jasco J-815 spectropolarimeter
- MuSIS 2007 multispectral image analysis system
- Leica DMS300 magnifier
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Raman spectroscopy
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We have:
- LabRam HR800 Raman and photoluminescence microscope.
- Jobin Yvon T64000 Raman high spectral resolution micro/macro with low frequency setting.
- Jobin Yvon VisHead Raman fibre optic analysis system at 785 nm with low frequency setting
- Linkam re-cooling and heating stage (-196 and 600 ºC)
- 5 lasers (325 nm, 514 nm, 532 nm, 785 nm, 1064 nm)
- ULF module to access to ultra-low Raman frequency regions between 5 and 100 cm-1
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Magnetic measurements
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We have:
- Bruker ESP300E electron paramagnetic resonance spectrometer.
- Quantum Design MPMS XL SQUID magnetometer.
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Polymorphism and calorimetry
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We have:
- Mettler Toledo DSC-822e/400 differential scanning calorimeter
- Mettler Toledo 851e/SF/1100 TGA-SDTA thermobalance
- Microcal VP-ITC isothermal trituration microcalorimeter
- Linkam/Nikon hot-stage thermomicroscope
- Surface Measurement Systems DVS-1000 Dynamic Vapor Sorption
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Nuclear magnetic resonance (NMR)
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We have:
- Bruker Avance Neo NMR spectrometer (800 MHz) with cryoprobe.
- Bruker Avance III NMR spectrometer (600 MHz) with cryoprobe.
- Varian VNMRS NMR spectrometer (500 MHz).
- 2 Varian Mercury NMR spectrometers (400 MHz).
- Bruker Avance III HD NMR spectrometer (400 MHz) with cryoprobe.
- Bruker Avance Neo NMR spectrometer (500 MHz).
- Bruker Avance Neo NMR spectrometer (400 MHz).
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Environment
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We have:
- TOC|MULTI N/C 3100 element analyser (Analytic Jena)
- Liquid chromatograph:
- Waters 515 HPLC pump
- IC-PAC anion column
- Kontron Waters 432 and UV-VIS conductivity detector
- Crison 522 conductimeter
- Microdrill model MicroMill (Electro Scientific Lasers)
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Separative techniques (HPLC, HPLC-MS and HPLC-HRMS)
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We have:
- Liquid chromatography (HPLC).
- Chromatographic systems Waters 600, Waters Alliance 2695, Waters Alliance 2795 coupled to absorbance detectors (UV-VIS and DAD), fluorescence, refraction index, light dispersion (evaporative), electrochemical and electrical conductivity detectors.
- Agilent HP 1100 chromatograph with DAD detector.
- UHPLC Acquity chromatograph with DAD detector.
- Jasco HPLC 2000 chromatograph with UV-VIS detector and electrical conductivity.
- Biochrom 30 chromatograph with post column derivatization with ninhydrin for the analysis of amino acids.
- Low resolution mass spectrometry coupled with liquid chromatography (HPLC-MS).
- 4000 QTRAP (AB SCIEX) triple quadrupole mass spectrometer coupled to Waters Acquity UPLC chromatograph.
- AB SCIEX 6500 QTRAP triple quadrupole mass spectrometer coupled to Agilent 1290 Infinity UHPLC chromatograph.
- SCIEX 7500 QTRAP triple quadrupole mass spectrometer coupled to Agilent 1290 Infinity II UHPLC.
- High resolution mass spectrometry coupled with liquid chromatography (HPLC-HRMS).
- LTQ-Orbitap Velos (Thermo Fisher Scientific) mass spectrometer equipped with an ion trap and Fourier transform analyzer, coupled to Ultimate 3000 (Thermo Fisher Scientific) UHPLC chromatograph
- QTOF Agilent 6560 mass spectrometer equipped with ionic mobility cell, coupled to Agilent 1290 Infinity II UHPLC chromatograph.
- Orbitrap IQ-X (Thermo Scientific) mass spectrometer equipped with an ion trap, quadrupole and Fourier transform analyzer, coupled to Vanquish Flex (Thermo Scientific) UHPLC chromatograph.
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