Centres Científics i Tecnològics UB

Equipment

Technologies

Bioscience Materials Chemical
· Image analysis · Surface analysis (ESCA / Auger) · Metal analysis (ICP-MS, ICP-OES, XRF, AA)
· Molecular interaction analysis · X-ray diffraction (XRD) · Applied gas chromatography–mass spectrometry
· Cytometry · Mechanical, electronic, and vacuum technology · Radiocarbon dating
· Electron cryomicroscopy · Molecular characterization mass spectrometry · Separative techniques (HPLC, LC-MS and LC-HRMS)
· Animal Experimentation Facility · Transmission electron microscope (TEM) applied to materials · Isotope-ratio mass spectrometry (IRMS)
· Genomics · SPM nano-techniques (interferometric-confocal microscopy, AFM, STM) · Molecular spectroscopy
· Electronic microscopy (TEM/SEM) · Scanning electron microscopy · Raman spectroscopy
· Advanced optical microscopy · Electron microprobe · Magnetic measurements
· Proteomics · Paleomagnetism · Polymorphism and calorimetry
· SPM nanobioscience techniques (AFM, STM) · Surface specific BET and porosimetry · Nuclear magnetic resonance (NMR)
· Technical unit for radiological protection · Environment

Equipaments

Image analysis We have:
  • Olympus BH2-UMA optical microscope connected to a Nikon D70 digital camera.
  • Digital camera.
  • Collection column.
  • Workstations.
Molecular interaction
analysis 		We have:
  • Biacore T200 (Cytiva).
Cytometry We have:

Bellvitge Campus:
  • Beckman Coulter MoFlo Astrios separator cytometer.
  • Becton Dickinson FACSCanto II analyser cytometer.
  • FACScalibur analyser cytometer with two lasers: 488 and 633.
  • Beckman Coulter Gallios analyser cytometer, 3 lasers, 10 colours.
  • Fluostar optima microplate reader (bmg) with double injection system and temperature regulator.
Diagonal Campus:
  • Mass cytometer (Cytof) Helios (Fluidigm).
  • Aria FUSION (Becton Dikinson) cell sorter, with flow hood, 4 lasers, 16 colors.
  • Aria SORP (Becton Dikinson) cell sorter, 4 lasers, 18 colors.
  • Gallios (Beckman Coulter) flow cytometer, 3 lasers, 10 colors.
  • Aurora (Cytek) flow cytometer, 4 lasers, spectral.
  • Magpix (Luminex)for soluble antigens detection.
Electron cryomicroscopy We have:
  • Spirit TWIN (FEI) 120 kV LaB6 Tecnai TEM cryomicroscope with Megaview 1kx1k CCD and tomograph.
  • Tecnai G2 F20 (FEI) 200 kV FEG TEM cryomicroscope with Eagle 4kx4k CCD and tomograph.
  • Jeol JEM 1010 100 kV TEM microscope with Megaview 1kx1k CCD.
  • Leica DM2000LED fluorescence microscope.
  • Leica UC6/FC6 cryoultramicrotome with cryosphere.
  • Leica UCT ultramicrotome.
  • Ultracut E ultramicrotome.
  • Leica UC7/FC7 cryoultramicrotome.
  • Vitrobot Mark III (FEI) immersion cryofixation/vitrification .
  • Leica AFS cryosubstitution (2).
  • Leica EM-AFS2 freeze substitution.
  • Baltec 060 BAF cryofracture/freeze-etching.
  • In situ laboratory: paraffin microtome, optical microscope and accessories.
  • Leica EM HPM100 high-pressure freezing.
  • Leica EM-ACE 600 high-vacuum carbon coater.
  • Modular Stereomicroscope Leica MZ6 with SFL 100 (fluorescence module).
  • Eppendorf 5804R refrigerated centrifuge.
Animal Experimentation Facility We have:
  • Cages and stable stalls.
  • Glass aquaria and polyester tanks.
  • Biological safety cabinets.
  • Extractor hoods.
  • Centrifuges.
  • Experimental operating room.
  • Surgical magnifying glasses.
  • Ventilators for assisted breathing.
  • Gas anaesthesia equipment.
  • Perkin Elmer In vivo IVIS-Lumina III Bioluminescence and Fluorescence equipment.
  • Hamamatsu Aequoria Bioluminescence and Fluorescence equipment.
  • Rodent stabling under conventional conditions.
  • Rodent stabling under SPF conditions.
  • Rodent stabling under negative pressure conditions.
  • Rabbit stabling under conventional conditions.
  • Pig stabling under conventional conditions.
  • Experimental labs in the conventional zone.
  • Experimental labs in the SPF zone.
  • Behavior labs in the conventional zone.
  • Behavior labs in the SPF zone.
  • Ventilated racks.
  • Filter cages.
  • Metabolic cages.
  • Confocal Laser Scanning Microscope and Multiphoton with Resonant Scanner. With temperature control.
  • 7T Experimental Magnetic Resonance imaging equipment.
  • Biological irradiator.
  • Software with tracking system SMART 3.0.
  • Thermal blankets.
  • Triple beam balance.
  • Precision balance.
  • Hydrogen peroxide SAS.
  • UV SAS.
  • Air shower.
  • ISO 9001:2015 quality management system.
  • AniBio, Unit management software.
Genomics We have:

Bellvitge Campus:
  • Applied Biosystems ABI Prism 7900 HT quantitative real-time PCR.
  • Chemagen Magnetic nucleic acid isolation module.
  • Agilent Technologies Bioanalyzer 2100.
  • BMG Labtech Fluostar Optima Microplate reader with double injection system and temperature control.
Diagonal Campus:
  • Applied Biosystems ABI Prism 3730 capillary electrophoresis DNA sequencer (48 capillary).
  • Applied Biosystems ABI Prism 3730XL capillary electrophoresis DNA sequencer (96 capillary).
  • Applied Biosystems ABI Prism 7900 HT quantitative real-time PCR.
  • Roche Light Cycler 480 II quantitative real-time PCR.
  • Applied Biosystems thermocyclers: PCR ABI 9700, PCR ABI 9800 Fast, PCR ABI 2400 and 2720; MGW Biotech PCR Primus thermocycler; Techne PCR TC-412 thermocycler.
  • Beckman Coulter Biomeck NXP robotic workstation.
  • Qiagen Mixer Mill MM 300 for grinding and homogenizing samples .
Electronic microscopy (TEM/SEM) We have:

Transmission electron microscope (TEM):
  • Transmission electron microscope JEOL 1010 + Orius (Gatan) CCD camera.
  • Reichert-Jung Ultracut E and Ultracut ultramicrotomes.
  • Optical microscope OLYMPUS CX41RF with OLYMPUS DP70 digital camera.
  • Glass knife machine Leica EMKMR2.
  • pyramidotom Reichert TM 60 and Leica EM TRIM.
  • Leica UC7 ultramicrotome with digital IC90-E camera.
Scanning electron microscope (SEM):
  • Scanning electron microscope JEOL JSM-7001F with secondary and retro-dispersed electron detectors.
  • Polaron Critical Point Drying and and Emitech Critical Point Drying.
  • Fisons Coating System SEM.
Advanced optical microscopy We have:
  • Culture room.
  • Fiji, Image J, MetaMorph, Imaris and Huygens image treatment workstations.
Diagonal Campus:
  • Leica CM3050S cryostat
  • Leica DMIRB wide-field transmitted light and fluorescence microscope.
  • Leica MLZIII stereo fluorescence microscope.
  • Leica RM 2135 microtome
  • Leica SPII confocal microscope with spectral detection.
  • Leica TCS-SPE spectral confocal microscope.
  • Leica Thunder high-speed fluorescence microscope.
  • Zeiss LSM880 high speed spectral confocal microscope.
  • Ted-Pella XH-4002 paraffin dispenser.
Campus Clínic:
  • Leica TCS-SP5 high speed, multiphoton, spectral confocal microscope.
  • Leica DMI6000 B wide-field fluorescence microscope.
  • Leica ASTP microinjector.
  • Zeiss LSM880 high speed spectral confocal microscope.
Bellvitge Campus:
  • Leica TCS-SL confocal microscope with spectral detection.
  • Carl Zeiss SteREO Lumar V12 stereo fluorescence microscope.
  • Nikon E800 Microscope.
  • Leica DMIRB Microscope.
  • Zeiss LSM880 high speed spectral confocal microscope.
Proteomics We have:

Electrophoresis:
  • Amersham Biosciences IPGphor 2-D electrophoresis protein separation system and Bio-Rad PROTEAN-IEF
Image analysis:
  • Amersham Biosciences DeCyder DIGE enabled TYPHOON TRIO fluorescent scanner.
Scission and digestion of gel stains:
  • Genomic Solutions Investigator ProGest digestion unit (up to 96 samples).
Mass spectrometry and liquid chromatography:
  • LTQ-Orbitrap Velos ion trap mass spectrometer with Thermo Finnigan electron transfer dissociation (ETD) accessory.
  • Thermo Finnigan LCQ DECA XP ESI ion trap.
  • Applied Biosystems MALDI-TOF Voyager DE perspective mass spectrometer.
  • Proxeon Nano LC Easy and Eksigent Nano LC Ultra AS2 nano liquid chromatographs.
Data analysis:
  • Licences for Protein Prospector, SEQUEST and Proteome Discoverer 1.2 from Thermo Finnigan , and PEAKS from BioInformatics Solutions.
SPM nanobioscience techniques (AFM, STM) We have:
  • Atomic force microscopes (AFM):
    • Bruker Multimode 8 with Nanoscope V controller electronics.
    • Bruker Dimension 3100 with Nanoscope IV controller electronics.

Technical unit for radiological protection We have:
  • Biological irradiator of animals and cells Shepherd Mark I30.
  • Wallac models 1209 and 1409 liquid scintillation counters.
  • Packard models 1500, 2100, 2810 and 2900 liquid scintillation counters.
  • Wallac Compugamma solid scintillation gamma counters.
  • Packard Cobra II solid scintillation gamma counters.
  • Biorad Molecular Imager FX and FX Pro Plus electronic autoradiography units.
  • Molecular Dynamics Typhoon 8600 electronic autoradiography unit.
  • FAG Contamat FHT-111M surface contamination measuring unit.
  • Thermo Scientific (RadEye) NE Technology PDM1A radiation level measuring equipment.
  • Atomtex AT1123 X-ray radiation level measuring equipment.
  • RTI Electronics Black Piranha,ETR1,Sedentex mannequin, etc. quality control equipment for X-ray units.
  • Hewlett Packard Faxitron 43855 A X-ray booth.
  • Fuji Medical Film FPM 100A automatic radiographic film developer.
  • Rad Elec Inc (Electets E-Perm) radon measurement system equipament in air.
  • Specific equipment for the management of radioactive waste and radiological protection.
Surface analysis (ESCA / Auger) We have:
  • PHI ESCA-5500 photoelectron spectroscope.
  • SPECS SAGE-HR photoelectron spectroscope .
  • SAM-670 PHI Auger microscope.
X-ray diffraction (XRD) We have:
  • PANalytical X’Pert PRO MRD diffractometer; high-resolution texture goniometer; parallel and mono-capillary optics; Bartels monochromator; PIXcel detector .
  • PANalytical X’Pert PRO MPD theta/theta diffractometer; reflection and transmission configurations; 45-position sample loader; Oxford Cryosystems 700 Cryostream series cryostat; PIXcel detector.
  • PANalytical X’Pert PRO MPD alpha1 diffractometer; focusing primary monochromator; 15-position sample loader; X’Celerator detector.
  • PANalytical X’Pert PRO MPD theta/theta diffractometer; Bragg-Bentano configuration; A PAAR HTK1200 high-temperature chamber; X’Celerator detector.
  • INEL CPS-120 diffractometer; 120º curved position detector; primary monochromator and parabolic mirror; FURCAP INEL temperature chamber .
  • Siemens D-500 diffractometers; grazing incidence and divergent optics; A PAAR TTK temperature chamber; scintillation detectors; Braun PSD position detector.
  • X-ray single crystal diffractometer Bruker D8 Venture. Cu and Mo microbeam sources. 4 circles Kappa goniometer. Integrated CCD video microscope. CMOS area detector Photon 100. Bruker Kryoflex II cryostat (80 - 400 K)
Granulometry We have:
  • Beckman Coulter LS 13 320 laser-diffraction particle dimension analyser.
Transmission electron microscope (TEM) applied to materials We have:
  • JEOL JEM 2100 transmission electron microscope with accessories for EDX analysis, STEM annular dark field, precession and tomographic detectors.
  • Philips CM30 300 kV transmission electron microscope.
  • Hitachi 800MT electron microscope with Gatan multiscan chamber .
  • JEOL JEM 2010F 200 kV transmission electron microscope with field emission tube with electron energy loss spectrometer (EELS) and image filter (GIF) and Z-STEM unit with high-angle annular detector.
SPM nano-techniques (interferometric-confocal microscopy, AFM, STM) We have:

  • Atomic force microscopy (AFM):
    • Bruker Multimode 8 with Nanoscope V controller electronics.
    • Bruker Dimension 3100 with Nanoscope IV controller electronics.
  • Leica DCM3D interferometric / confocal microscope.
Scanning electron microscopy We have:
  • Quanta 200 FEI, XTE 325/D8395 environmental scanning electron microscope.
  • Jeol J-6510 scanning electron microscope (Freezing unit, GATAN ALTO-1000, EDS detector, backscattered electrons detector).
  • JEDL J-7100 field emission scanning electron microscope (Cathodeluminiscence spectrometer GATAN MONO-CL4, EDS detector, retroscattered electron detector).
Electron microprobe We have:
  • JEOL JXA-8230 microprobe (one EDS and five WDS spectrometers).
Paleomagnetism We have:
  • 2G Enterprises 2G SRM755R superconducting rock magnetometer.
  • JR6A (Agico) spinner magnetometer.
  • TD48EU (ASC Scientific) thermal demagnetizer.
  • Magnetic Measurements Ltd. MMDT80 thermal demagnetizer.
  • Schonstedt Instruments TSD1 thermal demagnetizer.
  • Schonstedt Instruments GSD-5 alternating field demagnetizer.
  • ASC IM10-30 impulse magnetizer.
  • Geofyzica Brno KLY-2 magnetic susceptibilimeter.
Surface specific BET and porosimetry We have:
  • Porosimeter and BET determination of specific surface Micrometrics model Tristar 3000.
  • Accupic 1330 helium pycnometer.
Mechanical, electronic, and vacuum technology We have:
  • Goratu Group Lagun FU-TV 1250 and Lagun GVM 5-SP universal milling machines equipped with length displays on all three axes and precision accessories .
  • Pinacho S-90/225 lathe equipped with display, able to work with diameters of 400 mm.
  • Harrison M-300 lathe .
  • Metal shears for cutting sheet metal .
  • HB320SV drilling saw and saw for flat surfaces .
  • Lincoln Electric Invertec V200-T welding equipment .
  • Erlo-SPV drill with movable bench .
  • Precision and measurement tools.
Metal analysis (ICP-MS, ICP-OES, XRF, AA) We have:
  • Agilent 7500ce ICP-MS spectrometer with reaction cell.
  • Milestone DMA-80 direct mercury analyzer.
  • Perkin-Elmer Nexion 350d ICP-MS spectrometer with reaction cell.
  • Perkin-Elmer Elan-6000 ICP-MS spectrometer.
  • Perkin Elmer Optima 3200 RL simultaneous ICP-OES spectrometer.
  • Perkin Elmer Optima 8300 simultaneous ICP-OES spectrometer.
  • ICP-MS multi-collector Nu Instruments Plasma 3 spectrometer.
  • Panalytical, Axios PW 4400/40 sequential wavelength-dispersive X-ray spectrophotometer (WDXRF).
  • Varian AA240FS atomic absorption spectrophotometer with flame.
  • Multi-Collector Coupled Induction Plasma Mass Spectrometer (MC-ICPMS) Nu Instruments Plasma 3.
  • Aridus II desolvator sampling system .
Applied gas chromatography–mass spectrometry We have:
  • Thermo Fisher Scientific TRACE GC Ultra gas chromatograph coupled to an ITQU 900 (250 It)-Upgrade MS/MS mass spectrometer.
  • Thermo Fisher Scientific TRACE gas chromatograph coupled to a DSQII mass spectrometer with a TriPlus automatic injector headspace module .
  • Thermo Fisher Scientific Focus GC-DSQII gas chromatograph with a Markes International Unity Series II thermal desorption system.
  • Shimadzu QP2010 gas chromatograph coupled to a mass spectrometer.
  • Agilent Technologies 6890N gas chromatograph with a 5975 Inert XL mass spectrometer.
  • Agilent Technologies 5973 mass spectrometer with a direct introduction probe.
  • GC2025 gas chromatograph with FID detector (Shimadzu)
  • ThermoFisher Scientific TRACE 1300 chromatograph with detectors ECD and TCD
  • 7890A gas chromatograph with 5975C Inert XL mass spectrometer (Agilent Technologies). Gerstel autosampler (injectors cis,TDU, HS, PTV)
  • Cryogenic mill SPEX Certiprep 6850.
Radiocarbon dating We have:
  • Combustion tube under an oxygen stream.
  • Combustion pump under pressurized oxygen.
  • Chemical system for obtaining carbon dioxide by wet basis, acid decomposition or oxidation.
  • Chemical system for purifying carbon dioxide.
  • Chemical system for reducing carbon dioxide to benzene.
  • LKB-Wallac Rackbeta 1217 liquid scintillation counter.
  • Packard LL/TL 2255 liquid scintillation counter.
Molecular characterization mass spectrometry We have:
  • ABSciex MALDI TOF/TOF 4800 mass spectrometer.
  • Agilent Technologies LC/MSD TOF mass spectrometer coupled to an Agilent 1100 HPLC system.
Isotope-ratio mass spectrometry (IRMS) We have:
  • Finnigan MAT 253 isotope-ratio mass spectrometer with a Kiel IV carbonate device (ThermoFisher Scientific).
  • Finnigan MAT 252 isotope-ratio mass spectrometer with a Kiel III carbonate device.
  • Delta Plus XP isotope-ratio mass spectrometer with a TC-EA pyrolizer and a Carlo Erba 1108 element analyzer.
  • Delta Plus XP isotope-ratio mass spectrometer with a Gas Bench system, preconcentrator (Precon) and a Pal automatic sampler (ThermoFisher Scientific).
  • Picarro L2130i with a vaporizer HDS-2412.
  • MicroMill microtaladre (Electro Scientific Lasers).
  • Delta V Advantage isotope-ratio mass spectrometer with a Flash IRMS elemental analyzer and CONFLO IV interfaces (Thermo Fisher Scientific).
  • Delta Plus isotope-ratio mass spectrometer with a Agilent 6890 gas chromatograph and a Finnigan MAT Combustion III GC interface.
  • Delta V Advantage isotope-ratio mass spectrometer with a Trace GC gas chromatograph and a Surveyor liquid chromatograph and GC Isolink/LC Isolink interfaces (Thermo Fisher Scientific).
Molecular spectroscopy We have:
  • Analytik Jena Specord 205 UV-VIS spectrophotometer
  • Thermo IN10MX Fourier transform infrared spectrometer with imaging microscope
  • Perkin Elmer Frontier Fourier transform infrared spectrometer
  • Jasco J-815 spectropolarimeter
  • MuSIS 2007 multispectral image analysis system
  • Leica DMS300 magnifier
Raman spectroscopy We have:
  • LabRam HR800 Raman and photoluminescence microscope.
  • Jobin Yvon T64000 Raman high spectral resolution micro/macro with low frequency setting.
  • Jobin Yvon VisHead Raman fibre optic analysis system at 785 nm with low frequency setting
  • Linkam re-cooling and heating stage (-196 and 600 ºC)
  • 5 lasers (325 nm, 514 nm, 532 nm, 785 nm, 1064 nm)
  • ULF module to access to ultra-low Raman frequency regions between 5 and 100 cm-1
Magnetic measurements We have:
  • Bruker ESP300E electron paramagnetic resonance spectrometer.
  • Quantum Design MPMS XL SQUID magnetometer.
Polymorphism and calorimetry We have:
  • Mettler Toledo DSC-822e/400 differential scanning calorimeter
  • Mettler Toledo 851e/SF/1100 TGA-SDTA thermobalance
  • Microcal VP-ITC isothermal trituration microcalorimeter
  • Linkam/Nikon hot-stage thermomicroscope
  • Surface Measurement Systems DVS-1000 Dynamic Vapor Sorption
Nuclear magnetic resonance (NMR) We have:
  • Bruker Avance Neo NMR spectrometer (800 MHz) with cryoprobe.
  • Bruker Avance III NMR spectrometer (600 MHz) with cryoprobe.
  • Varian VNMRS NMR spectrometer (500 MHz).
  • 2 Varian Mercury NMR spectrometers (400 MHz).
  • Bruker Avance III HD NMR spectrometer (400 MHz) with cryoprobe.
  • Bruker Avance Neo NMR spectrometer (500 MHz).
  • Bruker Avance Neo NMR spectrometer (400 MHz).
Environment We have:
  • Analytik Jena Specord 205 UV-VIS spectrophotometer.
  • Crison 522 conductimeter.
  • Titrino Metrohm 702 SM automatic titrator.
  • Liquid chromatograph:
    • Waters 515 HPLC pump.
    • IC-PAC anion column.
    • Kontron Waters 432 and UV-VIS conductivity detector.
  • Analytic Jena Multi N/C 3100 TOC.
  • IKA C5003 element analyser and calorimetric chamber.
Separative techniques
(HPLC, HPLC-MS and HPLC-HRMS) 		We have:
  • High-resolution liquid chromatography :
    • Pumping systems: Waters 600, Waters Alliance 2695, Waters Alliance 2795, Jasco HPLC 2000, Agilent 1200 RR, Waters UPLC Acquity, Agilent HP 1100, Ultimate 3000 UHPLC Thermo, Agilent 1290 Infinity UHPLC and Agilent 1260 Infinity.
    • Detectors: Absorbance (ultraviolet-visible), fluorescence, refraction index, light dispersion (evaporative), electrochemical and electric conductivity.
    • Mass spectrometers: AB Sciex API 3000 QQQ, Thermo LTQ Orbitrap Velos, AB SCIEX 4000 QTRAP i AB SCIEX QTRAP 6500.
    • Acquisition and treatment of chromatographic data: Waters Empower, Agilent ChemStation, AB Sciex Analyst, Thermo Xcalibur and Jasco ChromNAV.
    • Q-TOF mass spectrometer with ionic mobility.
  • Amino acid analysers: Biochrom 30 (IE + post column ninhydrin) and Waters 600 HPLC ( AccQ-Tag precolumn).