Centres Científics i Tecnològics UB

Equipment

Technologies

Bioscience Materials Chemical
· Image analysis · Surface analysis (ESCA / Auger) · Metal analysis (ICP-MS, ICP-OES, XRF, AA)
· Molecular interaction analysis · X-ray diffraction (XRD) · Applied gas chromatography–mass spectrometry
· Cytometry · Mechanical, electronic, and vacuum technology · Radiocarbon dating
· Electron cryomicroscopy · Molecular characterization mass spectrometry · Separative techniques (HPLC, LC-MS and LC-HRMS)
· Animal Experimentation Facility · Transmission electron microscope (TEM) applied to materials · Isotope-ratio mass spectrometry (IRMS)
· Genomics · SPM nano-techniques (interferometric-confocal microscopy, AFM, STM) · Molecular spectroscopy
· Electronic microscopy (TEM/SEM) · Scanning electron microscopy · Raman spectroscopy
· Advanced optical microscopy · Electron microprobe · Magnetic measurements
· Proteomics · Paleomagnetism · Polymorphism and calorimetry
· SPM nanobioscience techniques (AFM, STM) · Surface specific BET and porosimetry · Nuclear magnetic resonance (NMR)
· Technical unit for radiological protection · Environment

Equipaments

Image analysis We have:
  • Olympus BH2-UMA optical microscope connected to a Nikon D70 digital camera.
  • Digital camera.
  • Collection column.
  • Workstations.
Molecular interaction
analysis 		We have:
  • Biacore T200 (Cytiva).
Cytometry We have:

Bellvitge Campus:
  • Beckman Coulter MoFlo Astrios separator cytometer, 4 lasers, 14 colors.
  • Becton Dickinson FACSCanto II analyser cytometer, 2 lasers, 6 colors.
  • FACScalibur analyser cytometer, 2 lasers, 4 colors.
  • Beckman Coulter Gallios analyser cytometer, 3 lasers, 10 colours.
  • Fluostar optima microplate reader (bmg) with double injection system and temperature regulator.
Diagonal Campus:
  • Mass cytometer (Cytof) Helios (Fluidigm).
  • Aria FUSION (Becton Dikinson) cell sorter, with flow hood, 4 lasers, 16 colors.
  • Aria SORP (Becton Dikinson) cell sorter, 4 lasers, 18 colors.
  • Gallios (Beckman Coulter) flow cytometer, 3 lasers, 10 colors.
  • Aurora (Cytek) flow cytometer, 4 lasers, spectral.
  • Magpix (Luminex)for soluble antigens detection.
Electron cryomicroscopy We have:
  • Spirit TWIN (FEI) 120 kV LaB6 Tecnai TEM cryomicroscope with Megaview 1kx1k CCD and tomograph.
  • Tecnai G2 F20 (FEI) 200 kV FEG TEM cryomicroscope with Eagle 4kx4k CCD and tomograph.
  • Jeol JEM 1010 100 kV TEM microscope with Megaview 1kx1k CCD.
  • Leica DM2000LED fluorescence microscope.
  • Leica UC6/FC6 cryoultramicrotome with cryosphere.
  • Leica UCT ultramicrotome.
  • Ultracut E ultramicrotome.
  • Leica UC7/FC7 cryoultramicrotome.
  • Vitrobot Mark III (FEI) immersion cryofixation/vitrification .
  • Leica AFS cryosubstitution (2).
  • Leica EM-AFS2 freeze substitution.
  • Baltec 060 BAF cryofracture/freeze-etching.
  • In situ laboratory: paraffin microtome, optical microscope and accessories.
  • Leica EM HPM100 high-pressure freezing.
  • Leica EM-ACE 600 high-vacuum carbon coater.
  • Modular Stereomicroscope Leica MZ6 with SFL 100 (fluorescence module).
  • Eppendorf 5804R refrigerated centrifuge.
Animal Experimentation Facility We have:
  • Cages and stable stalls.
  • Glass aquaria and polyester tanks.
  • Biological safety cabinets.
  • Extractor hoods.
  • Centrifuges.
  • Experimental operating room.
  • Surgical magnifying glasses.
  • Ventilators for assisted breathing.
  • Gas anaesthesia equipment.
  • Perkin Elmer In vivo IVIS-Lumina III Bioluminescence and Fluorescence equipment.
  • Hamamatsu Aequoria Bioluminescence and Fluorescence equipment.
  • Rodent stabling under conventional conditions.
  • Rodent stabling under SPF conditions.
  • Rodent stabling under negative pressure conditions.
  • Rabbit stabling under conventional conditions.
  • Pig stabling under conventional conditions.
  • Experimental labs in the conventional zone.
  • Experimental labs in the SPF zone.
  • Behavior labs in the conventional zone.
  • Behavior labs in the SPF zone.
  • Ventilated racks.
  • Filter cages.
  • Metabolic cages.
  • Confocal Laser Scanning Microscope and Multiphoton with Resonant Scanner. With temperature control.
  • 7T Experimental Magnetic Resonance imaging equipment.
  • Biological irradiator.
  • Software with tracking system SMART 3.0.
  • Thermal blankets.
  • Triple beam balance.
  • Precision balance.
  • Hydrogen peroxide SAS.
  • UV SAS.
  • Air shower.
  • ISO 9001:2015 quality management system.
  • AniBio, Unit management software.
Genomics We have:

Bellvitge Campus:
  • Applied Biosystems ABI Prism 7900 HT quantitative real-time PCR.
  • Applied Biosystems QuantStudio 7 pro quantitative real-time PCR.
  • Chemagen Magnetic nucleic acid isolation module.
  • Agilent Technologies Bioanalyzer 2100.
  • BMG Labtech Fluostar Optima Microplate reader with double injection system and temperature control.
Diagonal Campus:
  • Applied Biosystems ABI Prism 3730 capillary electrophoresis DNA sequencer (48 capillary).
  • Applied Biosystems ABI Prism 3730XL capillary electrophoresis DNA sequencer (96 capillary).
  • Applied Biosystems ABI Prism 7900 HT quantitative real-time PCR.
  • Roche Light Cycler 480 II quantitative real-time PCR.
  • Applied Biosystems QuantStudio 7 pro quantitative real-time PCR.
  • Agilent Technologies Bioanalyzer 2100.
  • Applied Biosystems thermocyclers: PCR ABI 9700, PCR ABI 9800 Fast, PCR ABI 2400 and 2720; MGW Biotech PCR Primus thermocycler; Techne PCR TC-412 thermocycler.
  • Beckman Coulter Biomeck NXP robotic workstation.
  • Qiagen Mixer Mill MM 300 for grinding and homogenizing samples .
Electronic microscopy (TEM/SEM) We have:

Transmission electron microscope (TEM):
  • Transmission electron microscope JEOL 1010 + Orius (Gatan) CCD camera.
  • Ultracut E ultramicrotome.
  • Reichert-Jung Ultracut ultramicrotome.
  • Leica UC7 ultramicrotome with digital IC90-E camera.
Scanning electron microscope (SEM):
  • Scanning electron microscope JEOL JSM-7001F with secondary and retro-dispersed electron detectors.
  • Polaron Critical Point Drying and and Emitech Critical Point Drying.
  • Fisons Coating System SEM.
  • Q150Tplus (Quorum) carbon evaporator.
Preparation laboratory:
  • MIKRO 200R (Hettich) refrigerated centrifuge.
  • 13/13 Dr Autocal (Roebling) osmometer.
  • pH-2001 (Crison) pH-meter.
  • OLYMPUS CX41RF optical microscope with OLYMPUS DP70 digital camera.
  • MOTIC BA410E optical microscope with 3+ Moticam digital camera.
  • SCZ-T4P Leica binocular magnifier.
  • SMZ745T Nikon binocular magnifier.
Advanced optical microscopy We have:
  • Culture room.
  • Fiji, Image J, MetaMorph, Imaris and Huygens image treatment workstations.
Bellvitge Campus:
  • Axio Imager M2 Apotome 3 fluorescence microscope.
  • Carl Zeiss SteREO Lumar V12 stereo fluorescence microscope.
  • Leica DMIRB Microscope.
  • Leica TCS-SL confocal microscope with spectral detection.
  • Nikon E800 Microscope.
  • Zeiss LSM880 high speed spectral confocal microscope.
Campus Clínic:
  • Bio rad gene gun
  • Leica TCS-SP5 high speed, multiphoton, spectral confocal microscope.
  • Leica DMI6000 B wide-field fluorescence microscope.
  • Leica ASTP microinjector.
  • Leica MZFL III stereoscope (color camera).
  • Leica DMRB widefield microscope (color camera).
  • Olympus BX51 widefield microscope (color Ccmera).
  • Zeiss LSM880 high speed spectral confocal microscope.
Diagonal Campus:
  • Leica CM3050S cryostat
  • Leica DMIRB wide-field transmitted light and fluorescence microscope.
  • Leica MLZIII stereo fluorescence microscope.
  • Leica RM 2135 microtome
  • Leica SPII confocal microscope with spectral detection.
  • Leica TCS-SPE spectral confocal microscope.
  • Leica Thunder high-speed fluorescence microscope.
  • Zeiss LSM880 high speed spectral confocal microscope.
  • Ted-Pella XH-4002 paraffin dispenser.
Proteomics We have:

Electrophoresis:
  • Amersham Biosciences IPGphor 2-D electrophoresis protein separation system and Bio-Rad PROTEAN-IEF
Image analysis:
  • Amersham Biosciences DeCyder DIGE enabled TYPHOON TRIO fluorescent scanner.
Scission and digestion of gel stains:
  • Genomic Solutions Investigator ProGest digestion unit (up to 96 samples).
Mass spectrometry and liquid chromatography:
  • LTQ-Orbitrap Velos ion trap mass spectrometer with Thermo Finnigan electron transfer dissociation (ETD) accessory.
  • Thermo Finnigan LCQ DECA XP ESI ion trap.
  • Applied Biosystems MALDI-TOF Voyager DE perspective mass spectrometer.
  • Proxeon Nano LC Easy and Eksigent Nano LC Ultra AS2 nano liquid chromatographs.
Data analysis:
  • Licences for Protein Prospector, SEQUEST and Proteome Discoverer 1.2 from Thermo Finnigan , and PEAKS from BioInformatics Solutions.
SPM nanobioscience techniques (AFM, STM) We have:
  • Atomic force microscopes (AFM):
    • Bruker Multimode 8 with Nanoscope V controller electronics.
    • Bruker Dimension 3100 with Nanoscope IV controller electronics.

Technical unit for radiological protection We have:
  • Biological irradiator of animals and cells Shepherd Mark I30.
  • Hidex model 600 SLe liquid scintillation counters.
  • Wallac models 1209 and 1409 liquid scintillation counters.
  • Packard models 1500, 2100, 2810 and 2900 liquid scintillation counters.
  • Wallac Compugamma solid scintillation gamma counters.
  • Packard Cobra II solid scintillation gamma counters.
  • Biorad Molecular Imager FX and FX Pro Plus electronic autoradiography units.
  • Molecular Dynamics Typhoon 8600 electronic autoradiography unit.
  • FAG Contamat FHT-111M surface contamination measuring unit.
  • Thermo Scientific (RadEye) NE Technology PDM1A radiation level measuring equipment.
  • Atomtex AT1123 X-ray radiation level measuring equipment.
  • RTI Electronics Black Piranha,ETR1,Sedentex mannequin, etc. quality control equipment for X-ray units.
  • Hewlett Packard Faxitron 43855 A X-ray booth.
  • Fuji Medical Film FPM 100A automatic radiographic film developer.
  • Rad Elec Inc (Electets E-Perm) radon measurement system equipament in air.
  • Specific equipment for the management of radioactive waste and radiological protection.
Surface analysis (ESCA / Auger) We have:
  • PHI ESCA-5500 photoelectron spectroscope.
  • SPECS SAGE-HR photoelectron spectroscope .
  • SAM-670 PHI Auger microscope.
X-ray diffraction (XRD) We have:
  • PANalytical X’Pert PRO MRD diffractometer; high-resolution texture goniometer; parallel and mono-capillary optics; Bartels monochromator; PIXcel detector .
  • PANalytical X’Pert PRO MPD theta/theta diffractometer; reflection and transmission configurations; 45-position sample loader; Oxford Cryosystems 700 Cryostream series cryostat; PIXcel detector.
  • PANalytical X’Pert PRO MPD alpha1 diffractometer; focusing primary monochromator; 15-position sample loader; X’Celerator detector.
  • PANalytical X’Pert PRO MPD theta/theta diffractometer; Bragg-Bentano configuration; A PAAR HTK1200 high-temperature chamber; X’Celerator detector.
  • INEL CPS-120 diffractometer; 120º curved position detector; primary monochromator and parabolic mirror; FURCAP INEL temperature chamber .
  • Siemens D-500 diffractometers; grazing incidence and divergent optics; A PAAR TTK temperature chamber; scintillation detectors; Braun PSD position detector.
  • X-ray single crystal diffractometer Bruker D8 Venture. Cu and Mo microbeam sources. 4 circles Kappa goniometer. Integrated CCD video microscope. CMOS area detector Photon 100. Bruker Kryoflex II cryostat (80 - 400 K)
Granulometry We have:
  • Beckman Coulter LS 13 320 laser-diffraction particle dimension analyser.
Transmission electron microscope (TEM) applied to materials We have:
  • JEOL JEM 2100 transmission electron microscope with accessories for EDX analysis, STEM annular dark field, precession and tomographic detectors.
  • Philips CM30 300 kV transmission electron microscope.
  • Hitachi 800MT electron microscope with Gatan multiscan chamber .
  • JEOL JEM 2010F 200 kV transmission electron microscope with field emission tube with electron energy loss spectrometer (EELS) and image filter (GIF) and Z-STEM unit with high-angle annular detector.
SPM nano-techniques (interferometric-confocal microscopy, AFM, STM) We have:

  • Atomic force microscopy (AFM):
    • Bruker Multimode 8 with Nanoscope V controller electronics.
    • Bruker Dimension 3100 with Nanoscope IV controller electronics.
  • Leica DCM3D interferometric / confocal microscope.
Scanning electron microscopy We have:
  • Quanta 200 FEI, XTE 325/D8395 environmental scanning electron microscope.
  • Jeol J-6510 scanning electron microscope (Freezing unit, GATAN ALTO-1000, EDS detector, backscattered electrons detector).
  • JEDL J-7100 field emission scanning electron microscope (Cathodeluminiscence spectrometer GATAN MONO-CL4, EDS detector, retroscattered electron detector).
Electron microprobe We have:
  • JEOL JXA-8230 microprobe (one EDS and five WDS spectrometers).
Paleomagnetism We have:
  • 2G Enterprises 2G SRM755R superconducting rock magnetometer.
  • ASC IM10-30 impulse magnetizer.
  • Geofyzica Brno KLY-2 magnetic susceptibilimeter.
  • JR6A (Agico) spinner magnetometer.
  • Magnetic Measurements Ltd. MMDT80 thermal demagnetizer.
  • MFK2 Kappabridge with a CS4 temperature unit and a CS-L cryostat (AGICO).
  • Schonstedt Instruments TSD1 thermal demagnetizer.
  • Schonstedt Instruments GSD-5 alternating field demagnetizer.
  • TD48EU (ASC Scientific) thermal demagnetizer.
Surface specific BET and porosimetry We have:
  • Porosimeter and BET determination of specific surface Micrometrics model Tristar 3000.
  • Accupic 1330 helium pycnometer.
Mechanical, electronic, and vacuum technology We have:
  • Goratu Group Lagun FU-TV 1250 and Lagun GVM 5-SP universal milling machines equipped with length displays on all three axes and precision accessories .
  • Pinacho S-90/225 lathe equipped with display, able to work with diameters of 400 mm.
  • Harrison M-300 lathe .
  • Metal shears for cutting sheet metal .
  • HB320SV drilling saw and saw for flat surfaces .
  • Lincoln Electric Invertec V200-T welding equipment .
  • Erlo-SPV drill with movable bench .
  • Precision and measurement tools.
Metal analysis (ICP-MS, ICP-OES, XRF, AA) We have:
  • Agilent 7500ce ICP-MS spectrometer with reaction cell.
  • Milestone DMA-80 direct mercury analyzer.
  • Perkin-Elmer Nexion 350d ICP-MS spectrometer with reaction cell.
  • Perkin-Elmer Elan-6000 ICP-MS spectrometer.
  • Perkin Elmer Optima 3200 RL simultaneous ICP-OES spectrometer.
  • Perkin Elmer Optima 8300 simultaneous ICP-OES spectrometer.
  • ICP-MS multi-collector Nu Instruments Plasma 3 spectrometer.
  • Panalytical, Axios PW 4400/40 sequential wavelength-dispersive X-ray spectrophotometer (WDXRF).
  • Varian AA240FS atomic absorption spectrophotometer with flame.
  • Multi-Collector Coupled Induction Plasma Mass Spectrometer (MC-ICPMS) Nu Instruments Plasma 3.
  • Aridus II desolvator sampling system .
Applied gas chromatography–mass spectrometry We have:
  • QP2010 gas chromatograph coupled to mass spectrometer (Shimadzu)
  • TRACE GC Ultra gas chromatograph coupled to ITQ 900 (250 lt)-Upgrade MSMS mass spectrometer (Thermo Fisher Scientific)
  • TRACE gas chromatograph coupled to DSQII mass spectrometer (Thermo Fisher Scientific) with TRIPLUS automatic injector Head Space module
  • FocusGC–DSQII gas chromatograph (Thermo Fisher Scientific) with UNITY Series II thermal desorption system(Markes International)
  • 6890N gas chromatograph with a 5975 Inert XL mass spectrometer (Agilent Technologies)
  • 7890A gas chromatograph with a 5975C Inert XL mass spectrometer (Agilent Technologies). Gerstel autosampler (injectors cis,TDU, HS, PTV)
  • 5973 mass spectrometer with a direct introduction probe (Agilent Technologies)
  • GC2025 gas chromatograph with a FID detector (Shimadzu)
  • TRACE 1300 gas chromatograph with ECD and TCD detectors (ThermoFisher Scientific)
  • Cryogenic mill SPEX Certiprep 6850
  • Elemental analysers CE1108, Flash 2000 and Flash1112 (Thermo Scientific)
Radiocarbon dating We have:
  • Combustion tube under an oxygen stream.
  • Combustion pump under pressurized oxygen.
  • Chemical system for obtaining carbon dioxide by wet basis, acid decomposition or oxidation.
  • Chemical system for purifying carbon dioxide.
  • Chemical system for reducing carbon dioxide to benzene.
  • LKB-Wallac Rackbeta 1217 liquid scintillation counter.
  • Packard LL/TL 2255 liquid scintillation counter.
Molecular characterization mass spectrometry We have:
  • ABSciex MALDI TOF/TOF 4800 mass spectrometer.
  • Agilent Technologies LC/MSD TOF mass spectrometer coupled to an Agilent 1100 HPLC system.
Isotope-ratio mass spectrometry (IRMS) We have:
  • MAT 253 isotope-ratio mass spectrometer (ThermoFisher Scientific) with:
  • Kiel IV carbonate device
  • Gas Bench system, Precon preconcentrator and Pal automatic sampler
  • MAT 253 isotope-ratio mass spectrometer with Kiel III carbonate device
  • Delta Plus XP isotope-ratio mass spectrometer (ThermoFisher Scientific) with:
  • TC-EA pyrolizer with solid sampler
  • TC-EA pyrolizer with liquid sampler
  • Carlo Erba 1108 element analyzer
  • MAT 253Plus isotope-ratio mass spectrometer (ThermoFisher Scientific) with:
  • GC Trace 1310 gas chromatograph with Triplus RSH sampler and GC Isolink II and ConFloIV interfaces
  • Gas Bench system, Precon preconcentrator and Pal automatic sampler
  • Delta V Advantage isotope-ratio mass spectrometer with a Trace GC gas chromatograph and GCIsolink and ConFlo IV interfaces (ThermoFisher Scientific)
  • Delta V Advantage isotope-ratio mass spectrometer with a Flash IRMS element analyzer and a CONFLO IV interface (ThermoFischer Scientific)
  • Delta Plus XP (isotope-ratio mass spectrometer (ThermoFisher Scientific) with:
  • Agilent 6890 gas chromatograph abd GC Combustion III interfaces
  • Surveyor gas chromatograph and LC Isolink interfaces
  • Picarro L2130i analyzer with a H2O stable isotopes HDS-2412 vaporizer
Molecular spectroscopy We have:
  • Analytik Jena Specord 205 UV-VIS spectrophotometer
  • Thermo IN10MX Fourier transform infrared spectrometer with imaging microscope
  • Perkin Elmer Frontier Fourier transform infrared spectrometer
  • Jasco J-815 spectropolarimeter
  • MuSIS 2007 multispectral image analysis system
  • Leica DMS300 magnifier
Raman spectroscopy We have:
  • LabRam HR800 Raman and photoluminescence microscope.
  • Jobin Yvon T64000 Raman high spectral resolution micro/macro with low frequency setting.
  • Jobin Yvon VisHead Raman fibre optic analysis system at 785 nm with low frequency setting
  • Linkam re-cooling and heating stage (-196 and 600 ºC)
  • 5 lasers (325 nm, 514 nm, 532 nm, 785 nm, 1064 nm)
  • ULF module to access to ultra-low Raman frequency regions between 5 and 100 cm-1
Magnetic measurements We have:
  • Bruker ESP300E electron paramagnetic resonance spectrometer.
  • Quantum Design MPMS XL SQUID magnetometer.
Polymorphism and calorimetry We have:
  • Mettler Toledo DSC-822e/400 differential scanning calorimeter
  • Mettler Toledo 851e/SF/1100 TGA-SDTA thermobalance
  • Microcal VP-ITC isothermal trituration microcalorimeter
  • Linkam/Nikon hot-stage thermomicroscope
  • Surface Measurement Systems DVS-1000 Dynamic Vapor Sorption
Nuclear magnetic resonance (NMR) We have:
  • Bruker Avance Neo NMR spectrometer (800 MHz) with cryoprobe.
  • Bruker Avance III NMR spectrometer (600 MHz) with cryoprobe.
  • Varian VNMRS NMR spectrometer (500 MHz).
  • 2 Varian Mercury NMR spectrometers (400 MHz).
  • Bruker Avance III HD NMR spectrometer (400 MHz) with cryoprobe.
  • Bruker Avance Neo NMR spectrometer (500 MHz).
  • Bruker Avance Neo NMR spectrometer (400 MHz).
Environment We have:
  • TOC|MULTI N/C 3100 element analyser (Analytic Jena)
  • Liquid chromatograph:
  • Waters 515 HPLC pump
  • IC-PAC anion column
  • Kontron Waters 432 and UV-VIS conductivity detector
  • Crison 522 conductimeter
  • Microdrill model MicroMill (Electro Scientific Lasers)
Separative techniques
(HPLC, HPLC-MS and HPLC-HRMS) 		We have:
  • Liquid chromatography (HPLC).
    • Chromatographic systems Waters 600, Waters Alliance 2695, Waters Alliance 2795 coupled to absorbance detectors (UV-VIS and DAD), fluorescence, refraction index, light dispersion (evaporative), electrochemical and electrical conductivity detectors.
    • Agilent HP 1100 chromatograph with DAD detector.
    • UHPLC Acquity chromatograph with DAD detector.
    • Jasco HPLC 2000 chromatograph with UV-VIS detector and electrical conductivity.
    • Biochrom 30 chromatograph with post column derivatization with ninhydrin for the analysis of amino acids.
  • Low resolution mass spectrometry coupled with liquid chromatography (HPLC-MS).
    • 4000 QTRAP (AB SCIEX) triple quadrupole mass spectrometer coupled to Waters Acquity UPLC chromatograph.
    • AB SCIEX 6500 QTRAP triple quadrupole mass spectrometer coupled to Agilent 1290 Infinity UHPLC chromatograph.
    • SCIEX 7500 QTRAP triple quadrupole mass spectrometer coupled to Agilent 1290 Infinity II UHPLC.
  • High resolution mass spectrometry coupled with liquid chromatography (HPLC-HRMS).
    • LTQ-Orbitap Velos (Thermo Fisher Scientific) mass spectrometer equipped with an ion trap and Fourier transform analyzer, coupled to Ultimate 3000 (Thermo Fisher Scientific) UHPLC chromatograph
    • QTOF Agilent 6560 mass spectrometer equipped with ionic mobility cell, coupled to Agilent 1290 Infinity II UHPLC chromatograph.
    • Orbitrap IQ-X (Thermo Scientific) mass spectrometer equipped with an ion trap, quadrupole and Fourier transform analyzer, coupled to Vanquish Flex (Thermo Scientific) UHPLC chromatograph.