Cytometry

• Conventional and spectral flow cytometry
• Mass cytometry
• Cell purification (“sorting”)
• Soluble antigen detection (Luminex technology ©)

• Purification of cell populations: cloning, sorting of transfectants through flow cytometry (FACS) or magnetic separation (MACS).
• Immunophenotyping of cell populations.
• Studies of cell viability and proliferation; high-throughput cell screening.
• Analysis of the cell cycle and ploidy variations.
• Cell function: measurements of second messengers, free-radical production, enzymatic kinetics.
• Plate immunoassays on microparticles.
• Detection and counting of microorganism populations.

• Purification of tumor stem cells.
• Cloning and purification of cells on 96-well plates; hybridoma sorting.
• Monitoring of blood populations undergoing ex vivo pharmacological treatment.
• Detection of toxicity and apoptosis; drug screening using cell lines.
• Detection of soluble cytokines in serum.
• Detection and counting of micro-organism populations in water (sea water, water from purification plants, industrial waste water, etc.).
• High-complexity immunophenotyping panels: more than 40 simultaneous labels per sample.

      Practical information for use of cytometry lab CCiTUB placed at PCB.