Centres Científics i Tecnològics UB

Electron cryomicroscopy

Contact: insitu@ccit.ub.edu

Sample preparation techniques:
Sample fixation and embedding at room temperature: conventional technique
Cryofixation: high pressure, impact, propane or ethane immersion
Cryosubstitution and cryoembedding
Cryofracture, freeze-drying and deep etching
Ultramicrotomy: semithin and ultrathin, all types of resins
Cryosectioning: cryopreparation and cryoslicing
Vitreous cryosectioning
Staining and labelling techniques:
Negative and contrast staining
In situ hybridization
Observation and analysis techniques:
Ambient temperature transmission electron microscopy (100 kV-120 kV)
Electron cryomicroscopy (200 kV FEG)
Electron tomography and cryotomography (+70º/-70º single axis)
Three-dimensional reconstruction
Optical microscopy techniques:
Paraffin embedding
Paraffin microtomy
In situ hybridization
In situ PCR
Conventional ultrastructure studies of simple cells and tissues
Conventional ultrastructure studies of isolated particles, isolated organelles or small organisms
High ultrastructural preservation of cells, tissues, particle samples and small organisms by means of slicing; various degrees of preservation and visualization
Study of the organization of intramembrane particles
Topographic and/or interior nanometric characterization of lipo/lipoprotein compounds, proteins, proteins assemblies, membranes and biomaterials
Three-dimensional reconstruction of isolated or particle structures
In situ three-dimensional reconstruction of structures (macromolecules, nanomachines and subcellular bodies) in cell interiors
Molecular recognition or in situ localization at the subcellular level of proteins, nucleic acids and other macromolecules
Quantitative studies of measurements or labelling on the nanometric scale
Basic preparation for transmission optical microscopy
3D study of the structure of proteins and macromolecular complexes
3D study of cell structures
Characterization of liposomes, micelles, bicelles and other lipoprotein particles; effect of surfactants; interaction of surfactants with biological material as vehicles for drug delivery
Study of nanoparticles with peptides, proteins, etc. and their possible entry into tumour cells or through the skin, mucous membranes, haemato-encephalitic barrier, etc.
Study of functionalized cells or biomolecules on nanostructured substrates
3D studies of proteins of pharmacological interest as drug targets
Characterization of peptide hydrogels
Ultrastructural study of biological disease models, for example, the malaria parasite infecting the red blood cell or structural changes in tumour cells
Study of ultrastructural changes in cells caused by drug trials or genetic modifications of different types

Surname Name Location Phone number Fax Email
DELGADO VALDERRAMA (*) LIDIA Edifici Clúster (PCB) C/ Baldiri Reixac, 10 934034658/ 934034860 934039037 ldelgado@ccit.ub.edu
MARTINEZ RUIZ GEMMA MARTA Edifici Clúster (PCB) C/ Baldiri Reixac, 10 934034658/ 934034860 934039037 gema@ccit.ub.edu
MUELA CASTRO MARIA YOLANDA Edifici Clúster (PCB) C/ Baldiri Reixac, 10 934034658/ 934034860 934039037 yolanda@ccit.ub.edu

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