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Sample preparation techniques:
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Sample fixation and embedding at room temperature: conventional technique
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Cryofixation: high pressure, impact, propane or ethane immersion
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Cryosubstitution and cryoembedding
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Cryofracture, freeze-drying and deep etching
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Ultramicrotomy: semithin and ultrathin, all types of resins
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Cryosectioning: cryopreparation and cryoslicing
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Vitreous cryosectioning
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Staining and labelling techniques:
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Negative and contrast staining
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Immunolabelling
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In situ hybridization
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Autoradiography
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Observation and analysis techniques:
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Ambient temperature transmission electron microscopy (100 kV-120 kV)
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Electron cryomicroscopy (200 kV FEG)
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Electron tomography and cryotomography (+70º/-70º single axis)
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Three-dimensional reconstruction
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Optical microscopy techniques:
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Paraffin embedding
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Paraffin microtomy
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In situ hybridization
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In situ PCR
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Conventional ultrastructure studies of simple cells and tissues
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Conventional ultrastructure studies of isolated particles, isolated organelles or small organisms
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High ultrastructural preservation of cells, tissues, particle samples and small organisms by means of slicing; various degrees of preservation and visualization
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Study of the organization of intramembrane particles
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Topographic and/or interior nanometric characterization of lipo/lipoprotein compounds, proteins, proteins assemblies, membranes and biomaterials
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Three-dimensional reconstruction of isolated or particle structures
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In situ three-dimensional reconstruction of structures (macromolecules, nanomachines and subcellular bodies) in cell interiors
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Molecular recognition or in situ localization at the subcellular level of proteins, nucleic acids and other macromolecules
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Quantitative studies of measurements or labelling on the nanometric scale
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Basic preparation for transmission optical microscopy
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3D study of the structure of proteins and macromolecular complexes
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3D study of cell structures
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Characterization of liposomes, micelles, bicelles and other lipoprotein particles; effect of surfactants; interaction of surfactants with biological material as vehicles for drug delivery
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Study of nanoparticles with peptides, proteins, etc. and their possible entry into tumour cells or through the skin, mucous membranes, haemato-encephalitic barrier, etc.
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Study of functionalized cells or biomolecules on nanostructured substrates
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3D studies of proteins of pharmacological interest as drug targets
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Characterization of peptide hydrogels
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Ultrastructural study of biological disease models, for example, the malaria parasite infecting the red blood cell or structural changes in tumour cells
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Study of ultrastructural changes in cells caused by drug trials or genetic modifications of different types
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Surname
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Name
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Location
|
Phone number
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Email
|
|
DELGADO VALDERRAMA (*)
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LIDIA
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Edifici Clúster (PCB) C/ Baldiri Reixac, 10
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934034860
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ldelgado@ccit.ub.edu
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|
MUELA CASTRO
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MARIA YOLANDA
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Edifici Clúster (PCB) C/ Baldiri Reixac, 10
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934034860
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yolanda@ccit.ub.edu
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(*) for more information
|
