Centres Científics i Tecnològics UB

Electronic microscopy (TEM/SEM)

Contact: electromed@ccit.ub.edu

Transmission electron microscopy (TEM)
Fixation methods for biological samples: chemical and/or cryofixation
Dehydration and cryosubstitution techniques
Embedding and cryoembedding
Ultramicrotomy and cryoultramicrotomy techniques
Negative staining techniques for suspensions
Scanning electron microscopy (SEM)
Fixation methods for biological samples: chemical and/or cryofixation
Dehydration and cryosubstitution techniques
Critical point drying of samples and other chemical agents
Different types of assembly according to sample type
Optical microscopy
Semithin sections (0.5 – 3 µm) and specific stains
Molecular localization techniques
Immunolocalization techniques (markers: nanogold, gold, quantum dots, silver etc.)
Lectin techniques (localization of sugar residues)
Correlative techniques (Observation of the same sample with electronic and optical microscopes)
Transmission electron microscopy (TEM)
Ultrastructural, tissue, cellular and molecular studies
Studies of bacterial, viral, bacteriophagic and nitrogen suspensions, etc.
Ultrastructural studies of isolated cell organelles, mitochondrial pellets and chloroplasts
Whole-mount studies of cells and bacteria, etc.
Molecular localization and co-localization of proteins, glucidic residues, etc.
Cytochemical studies (Enzyme activity, digestion, etc.)
Optical microscope studies with semithin sections of samples enclosed in resin
Scanning electron microscopy (SEM)
Microstructural analysis of cell cultures, tissues and organs with 3-D imaging
Study of cell suspensions
Surface studies: bone, teeth, corneas skin, etc.
Study of biofilms and their formation on different surfaces
Morphological studies of small organisms and microorganisms
Molecular localization.
Study of biomaterials, implants, etc.
Study, analysis and evaluation of images obtained
Study of cell cultures and tissues
Study of fungal pathogens on plants
Study of the incorporation of markers inside cells
Study of human spermatozoa
Studies of biofilms on water filters and cultures, tracheal tubes, historic moments, etc.
Surface studies: prostheses removed from patients, presence of sperm on tissue
TEM localization of nanogold in different tissues absorbed via inhalation in live rats
Study of nanogold in textiles
Study of bacteriophagic contamination in the bacterial production of lactose
Study of protein aggregation in Alzheimer patients
Clinical biopsy processing

Surname Name Location Phone number Fax Email
GARCIA SOTO ALMUDENA Fac. Medicina C/ Casanova, 143 934034407 934034408 almudena@ccit.ub.edu
REBLED CORSELLAS (*) JOSEP MANEL Fac. Medicina C/ Casanova, 143 934034407 934034408 jmrebled@ccit.ub.edu
RIVERA DURAN ROSA Fac. Medicina C/ Casanova, 143 934034407 934034408 rosarivera@ccit.ub.edu
VILLUENDAS LATORRE ARANZAZU Fac. Medicina C/ Casanova, 143 934034407 934034408 aranzavila@ccit.ub.edu


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