Noticias08.07.2014Publicación del artículo "High-throughput sperm differential proteomics suggests that epigenetic alterations contribute to failed assisted reproduction" |
|
|
El doctor Josep Maria Estanyol, responsable de la Tecnología de Proteómica de los CCTiUB, ha participado conjuntamente con otros especialistas en el artículo "High-throughput sperm differential proteomics suggests that epigenetic alterations contribute to failed assisted reproduction" publicado en la edición de junio de la revista Human Reproduction. El abstract es el siguiente: "Normozoospermic sperm samples with different IVF outcomes (pregnancy versus no pregnancy) differed in the levels of at least 66 proteins. The analysis of the proteome of sperm samples with distinct fertilization capacity using low-throughput proteomic techniques resulted in the detection of a few differential proteins. Current high-throughput mass spectrometry approaches allow the identification and quantification of a substantially higher number of proteins. By using TMT labelling and LC-MS/MS, we have detected 31 proteins present at lower abundance (ratio no pregnancy/pregnancy < 0.67) and 35 at higher abundance (ratio no pregnancy/pregnancy > 1.5) in the no pregnancy group. Bioinformatic analyses showed that the proteins with differing abundance are involved in chromatin assembly and lipoprotein metabolism (P values < 0.05). In addition, the differential abundance of one of the proteins (SRSF protein kinase 1) was further validated by western blotting using independent samples (P value < 0.01)." El artículo completo se encuentra en el siguiente enlace. | |