News08.07.2014Publication of the paper "High-throughput sperm differential proteomics suggests that epigenetic alterations contribute to failed assisted reproduction" |
|
|
Dr. Josep Maria Estanyol, responsible of the Proteomics Technology of the CCTiUB, participated in the paper "High-throughput sperm differential proteomics suggests that epigenetic alterations contribute to failed assisted reproduction" published at the June Edition of the Human Reproduction Journal. The abstract: "Normozoospermic sperm samples with different IVF outcomes (pregnancy versus no pregnancy) differed in the levels of at least 66 proteins. The analysis of the proteome of sperm samples with distinct fertilization capacity using low-throughput proteomic techniques resulted in the detection of a few differential proteins. Current high-throughput mass spectrometry approaches allow the identification and quantification of a substantially higher number of proteins. By using TMT labelling and LC-MS/MS, we have detected 31 proteins present at lower abundance (ratio no pregnancy/pregnancy < 0.67) and 35 at higher abundance (ratio no pregnancy/pregnancy > 1.5) in the no pregnancy group. Bioinformatic analyses showed that the proteins with differing abundance are involved in chromatin assembly and lipoprotein metabolism (P values < 0.05). In addition, the differential abundance of one of the proteins (SRSF protein kinase 1) was further validated by western blotting using independent samples (P value < 0.01)." You can read the full paper in the following link. | |