Notícies
08.07.2014
|
|
|
El doctor Josep Maria Estanyol, responsable de la Tecnologia de Proteòmica dels CCTiUB, ha participat conjuntament amb altres especialistes en l'article "High-throughput sperm differential proteomics suggests that epigenetic alterations contribute to failed assisted reproduction" publicat a l'edició de juny de la revista Human Reproduction. L'abstract és el següent: "Normozoospermic sperm samples with different IVF outcomes (pregnancy versus no pregnancy) differed in the levels of at least 66 proteins. The analysis of the proteome of sperm samples with distinct fertilization capacity using low-throughput proteomic techniques resulted in the detection of a few differential proteins. Current high-throughput mass spectrometry approaches allow the identification and quantification of a substantially higher number of proteins. By using TMT labelling and LC-MS/MS, we have detected 31 proteins present at lower abundance (ratio no pregnancy/pregnancy < 0.67) and 35 at higher abundance (ratio no pregnancy/pregnancy > 1.5) in the no pregnancy group. Bioinformatic analyses showed that the proteins with differing abundance are involved in chromatin assembly and lipoprotein metabolism (P values < 0.05). In addition, the differential abundance of one of the proteins (SRSF protein kinase 1) was further validated by western blotting using independent samples (P value < 0.01)." L'article complet es troba al següent enllaç. | |