News
05.05.2021New service: Correlative Light-Electron Microscopy (CLEM)
The TEM-SEM Electron Microscopy and Advanced Optical Microscopy units located in the August Pi i Sunyer Medicine-Clinical Campus (both of the Scientific and Technological Centers of the University of Barcelona CCiTUB), thanks to the collaboration of the professors Carlos Enrich and Carlos Rentero from the UB Department of Biomedicine are in a position to offer the possibility of carrying out correlation experiments between both characterization techniques. The well-known CLEM technique (by its name: Correlative Light-Electron Microscopy) allows to carry out complementary studies working together with confocal fluorescence microscopy (CLSM, thanks to different fluorochromes for the localization of certain biomolecules) and transmission electron microscopy (TEM, obtaining images at high magnification, entering the nanoscale). The correlation of both results improves the understanding of cellular processes, relating functional expression with the structures where it occurs.
The images show TEM and CLSM images on the same HeLa cell that has been induced to accumulate free cholesterol in late endosomes and lysosomes (in green, by marking with anti-LBPA). The nucleus is shown in red (marked TOPRA-3) and free cholesterol in blue (Filipino staining). The inset shows the cellular compartments where the anti-LBPA label accumulates.
Images provided by Carles Enrich and Carles Rentero.