| Tuesday 16 February 2016 |
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| 09.30 | | Welcome. |
| 09:45 - 11:30 | | Lectures. |
| 11:30 - 12:00 | | Coffe break. |
| 12:00 - 13:00 | | Lectures. |
| 13:00 - 14:00 | | Lunch. |
| 14:00 - 19:00 | | Workshop 1: Chemotaxis ( part 1 ). |
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| Wednesday 17 February 2016 |
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| 09:30 - 11:30 | | Workshop 1: Chemotaxis ( part 2 ). |
| 11:30 - 12:00 | | Coffe break. |
| 12:00 - 13:00 | | Workshop 2: Perfusion ( part 1 ). |
| 13:00 - 14:00 | | Lunch |
| 14:00 - 16:00 | | Workshop 2: Perfusion ( part 2 ). |
Extended program |
LECTURES.
Tuesday 16 February 2016 |
| 9:45 - 11:30 | | |
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- Maria Calvo: Live Cell Imaging in Biomedical Research.
- Ulf Raedler: Cells in focus - new ways in cell microscopy.
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| 12:00 - 13:00 | | |
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- Elias Horn: Pioneering results with chemotaxis and perfusion assays.
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| WORKSHOP 1. CHEMOTAXIS |
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| Tuesday 16 February 2016. |
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| 14:00 - 15:00 | | Hands on Chemotaxis experiments: |
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- Filling gradient chemotaxis slides
- Food coloring
- Prepare the cells collect and count while
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| 15:00 - 16:30 | | Cell seeding in 2D and 3D Collagen Gel Matrices |
| 16:30 - 17:00 | | Coffe Break |
| 17:00 - 17:30 | | Filling gradient chemotaxis slides |
| 17:30 - 19:00 | | Automated Wide field and Confocal Microscopy for overnight experiments |
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| Wednesday 17 February 2016. |
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| 09:30 - 10:00 | | Overview of Chemotaxis over-night experiments on microscope. |
| 10:00 - 10:30 | | Talk: chemotaxis parameters descriptive parameters of migration. |
| 10:30 - 11:30 | | Tracking and Analysis. Software: ImageJ / plugins. |
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| WORKSHOP 2. PERFUSION |
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| Wednesday 17 February 2016. |
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| 12:00 - 13:00 | | Talk: Shear stress, pumps: how they work, types |
| 13:00 - 14:00 | | Lunch |
| 14:00 - 16:00 | | Hands on Perfusion assays: |
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- Handling of channel slides, cell seeding
- Exposing cells to flow
- Setup of ibidi GmbH Pump System Flow rate
- Measurements of flow rate
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