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LIVE CELL IMAGING OF CHEMOTAXIS AND PERFUSION ASSAYS

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Main topics
Program
Registration
Teachers
Organiza
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Objectives

Aimed at

Participants

Date

Location

Objectives

The aim of the workshop is to learn the experimental setup and analysis of chemotaxis and perfusion assays by means of live cell microscopy.

Aimed at

The workshop is intended for scientists and technical associates experienced in cell culture and microscopy who want to establish chemotaxis and/or perfusion experiments in their lab.

Number of participants

Talks:

70 participants

Practical sessions:

10 participants chemotaxis
10 participants perfusion

Talks will be at room 13 of School of Medicine:

School of Medicine
5th floor- Cloister
C/Casanova 143
Barcelona 08036

Workshops:

Advanced Optical Microscopy Unit
Scientific and Technological Centers
University of Barcelona
School of Medicine
5th floor- South Area
C/Casanova 143
Barcelona 08036

Main topics:

Chemotaxis Assays

Analysis of chemotaxis of adherent cells in 2D and non-adherent cells in 3D gel matrices.
Sample preparation
Cell tracking
Automated live cell microscopy
Descriptive parameters of cell migration
Data analysis and presentation of results
Experiments are performed and analyzed using HT-1080 cancer cells

Perfusion Assays

Analysis of Perfusion based assays
Physics of flow chambers
Sample preparation
Culture under physiological flow conditions
Data analysis and presentation of results
Endothelial cells are cultured under physiological flow conditions

Program:

Tuesday 16 February 2016

09.30		Welcome.
09:45 - 11:30		Lectures.
11:30 - 12:00		Coffe break.
12:00 - 13:00		Lectures.
13:00 - 14:00		Lunch.
14:00 - 19:00		Workshop 1: Chemotaxis ( part 1 ).



Wednesday 17 February 2016

09:30 - 11:30		Workshop 1: Chemotaxis ( part 2 ).
11:30 - 12:00		Coffe break.
12:00 - 13:00		Workshop 2: Perfusion ( part 1 ).
13:00 - 14:00		Lunch
14:00 - 16:00		Workshop 2: Perfusion ( part 2 ).
Extended program
LECTURES. Tuesday 16 February 2016
9:45 - 11:30
		- Maria Calvo: Live Cell Imaging in Biomedical Research. - Ulf Raedler: Cells in focus - new ways in cell microscopy.
12:00 - 13:00
		- Elias Horn: Pioneering results with chemotaxis and perfusion assays.



WORKSHOP 1. CHEMOTAXIS

Tuesday 16 February 2016.


14:00 - 15:00		Hands on Chemotaxis experiments:
		- Filling gradient chemotaxis slides - Food coloring - Prepare the cells collect and count while

15:00 - 16:30		Cell seeding in 2D and 3D Collagen Gel Matrices
16:30 - 17:00		Coffe Break
17:00 - 17:30		Filling gradient chemotaxis slides
17:30 - 19:00		Automated Wide field and Confocal Microscopy for overnight experiments



Wednesday 17 February 2016.


09:30 - 10:00		Overview of Chemotaxis over-night experiments on microscope.
10:00 - 10:30		Talk: chemotaxis parameters descriptive parameters of migration.
10:30 - 11:30		Tracking and Analysis. Software: ImageJ / plugins.



WORKSHOP 2. PERFUSION

Wednesday 17 February 2016.


12:00 - 13:00		Talk: Shear stress, pumps: how they work, types
13:00 - 14:00		Lunch
14:00 - 16:00		Hands on Perfusion assays:
		- Handling of channel slides, cell seeding - Exposing cells to flow - Setup of ibidi GmbH Pump System Flow rate - Measurements of flow rate

Registration form:

(*) mandatory fields

For more information contact to:
confomed@ccit.ub.edu.

Teachers:

Maria Calvo. Technology of Advanced Optical Microscopy of CCiTUB

Anna Bosch. Technology of Advanced Optical Microscopy of CCiTUB

Elisenda Coll. Technology of Advanced Optical Microscopy of CCiTUB

Ulf Raedler. ibidi GmbH

Elias Horn. ibidi GmbH

Organized by:
Centres Científics i Tecnològics Universitat de Barcelona Director: Dr. José Ramón Seoane Trigo Coordinator: Dra. Maria Calvo Technology of Advanced Optical Microscopy of CCiTUB.	ibidi GmbH