Advanced optical microscopy

• Wide-field fluorescence and transmitted optical microscopy.
• Confocal laser microscopy.
• Two-photon excitation microscopy (multiphoton).
• Second Harmonic Generation (SHG). Polarization SHG.
• Processing and analysis of images.
• Non invasive Bioluminescence imaging (rodents).
• Cell cultures and micromanipulation.
• Paraffin embedding.
• Microtomy.
• Cryosectioning.

• Fluorescence and transmitted light multichannel and multidimensional (xyztc) images.
• Multi-position and mosaic images.
• Live cell microscopy, short courses.
• Analysis of molecular proximity: fluorescence resonance energy transfer (FRET).
• Analysis of molecular dynamics: fluorescence recovery after photobleaching (FRAP), Inverse FRAP, fluorescence loss in photobleaching (FLIP) and photolabelling.
• Intravital microscopy on live animals (high speed multiphotons).
• Infrared optical microscopy: multiphoton and second-harmonic generation.
• Maintenance of cell lines for microscopy experiments.
• Cell micromanipulation: microinjection.
• Cellular and molecular labelling (transfection, staining).
• Immunocytochemistryç: Cellular and molecular labeling (transfections, staining).
• Immunohistochemistry: Tissues and organs processing and staining.
• Image analysis:
• Image processing (filtered, 3D reconstruction, video montages) and analysis (co-localization, molecular dynamics, particle and surface analysis).
• Development of instruction macros.
• Assistance in the design, preparation, performance and analysis of optical microscopy experiments.
• Theoretical and practical courses.

• Particle analysis.
• Molecular imaging for the study of cellular and molecular processes in vivo.
• Functionality of biosensors and drug delivery systems in cell cultures.
• Cytotoxicity and cell viability trials.
• Cell migration trials in 2D and 3D cultures.
• Quantification of bone-implant contact (BIC) in teeth.
• Morphometric analysis.
• Subcellular and kinetic localization of fluorescent pharmaceuticals.
• Neurite growth analysis.
• Colocalization analysis.
• Cell viability studies.
• Cellular uptake studies.
• Subcellular localization of molecules.
• Applied research in human samples and animal experimental models (rat, mouse, C. Elegans, Xenopus Tropicalis…).
• Live Cell tracking.
• Confocal profilometry.

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