Centres Científics i Tecnològics UB

Advanced optical microscopy

  • Wide-field fluorescence and transmitted optical microscopy.
  • Confocal laser microscopy.
  • Two-photon excitation microscopy (multiphoton).
  • Second Harmonic Generation (SHG). Polarization SHG.
  • Processing and analysis of images.
  • Non invasive Bioluminescence imaging (rodents).
  • Cell cultures and micromanipulation.
  • Paraffin embedding.
  • Microtomy.
  • Cryosectioning.
  • Fluorescence and transmitted light multichannel and multidimensional (xyztc) images.
  • Multi-position and mosaic images.
  • Live cell microscopy, short courses.
  • Analysis of molecular proximity: fluorescence resonance energy transfer (FRET).
  • Analysis of molecular dynamics: fluorescence recovery after photobleaching (FRAP), Inverse FRAP, fluorescence loss in photobleaching (FLIP) and photolabelling.
  • Intravital microscopy on live animals (high speed multiphotons).
  • Infrared optical microscopy: multiphoton and second-harmonic generation.
  • Maintenance of cell lines for microscopy experiments.
  • Cell micromanipulation: microinjection.
  • Cellular and molecular labelling (transfection, staining).
  • Immunocytochemistryç: Cellular and molecular labeling (transfections, staining).
  • Immunohistochemistry: Tissues and organs processing and staining.
  • Image analysis:
    • Image processing (filtered, 3D reconstruction, video montages) and analysis (co-localization, molecular dynamics, particle and surface analysis).
    • Development of instruction macros.
  • Assistance in the design, preparation, performance and analysis of optical microscopy experiments.
  • Theoretical and practical courses.
  • Particle analysis.
  • Molecular imaging for the study of cellular and molecular processes in vivo.
  • Functionality of biosensors and drug delivery systems in cell cultures.
  • Cytotoxicity and cell viability trials.
  • Cell migration trials in 2D and 3D cultures.
  • Quantification of bone-implant contact (BIC) in teeth.
  • Morphometric analysis.
  • Subcellular and kinetic localization of fluorescent pharmaceuticals.
  • Neurite growth analysis.
  • Colocalization analysis.
  • Cell viability studies.
  • Cellular uptake studies.
  • Subcellular localization of molecules.
  • Applied research in human samples and animal experimental models (rat, mouse, C. Elegans, Xenopus Tropicalis…).
  • Live Cell tracking.
  • Confocal profilometry.

Surname Name Location Phone number Email
BARROSO INDIANO BEATRIZ Bellvitge C/ Feixa Llarga s/n 934034863 bbarroso@ccit.ub.edu
BOSCH MARIMON (*) MANEL Fac. Biologia. Av. Diagonal, 645 934020176 mbosch@ccit.ub.edu
CALVO ADAMUZ (*) MARIA Fac. Medicina C/ Casanova, 143 934037159/
934039930
mcalvo@ccit.ub.edu
CASTAÑO BOLDU (*) ESTHER Bellvitge C/ Feixa Llarga s/n 934034863 ecastano@ccit.ub.edu
MARTÍN MALPARTIDA GEMMA Fac. Medicina C/ Casanova, 143 934037159 gemmamartin@ccit.ub.edu
TORREJON ESCRIBANO BENJAMIN Bellvitge C/ Feixa Llarga s/n 934034863 torrejonbenja@ccit.ub.edu
UNZUETA PAYERAS FERNANDO Fac. Medicina C/ Casanova, 143 934037159 funzueta@ccit.ub.edu

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