Electron cryomicroscopy

• Sample preparation techniques:
• Sample fixation and embedding at room temperature: conventional technique
• Cryofixation: high pressure, impact, propane or ethane immersion
• Cryosubstitution and cryoembedding
• Cryofracture, freeze-drying and deep etching
• Ultramicrotomy: semithin and ultrathin, all types of resins
• Cryosectioning: cryopreparation and cryoslicing
• Vitreous cryosectioning
• Staining and labelling techniques:
• Negative and contrast staining
• Immunolabelling
• In situ hybridization
• Autoradiography
• Observation and analysis techniques:
• Ambient temperature transmission electron microscopy (100 kV-120 kV)
• Electron cryomicroscopy (200 kV FEG)
• Electron tomography and cryotomography (+70º/-70º single axis)
• Three-dimensional reconstruction
• Optical microscopy techniques:
• Paraffin embedding
• Paraffin microtomy
• In situ hybridization
• In situ PCR

• Conventional ultrastructure studies of simple cells and tissues
• Conventional ultrastructure studies of isolated particles, isolated organelles or small organisms
• High ultrastructural preservation of cells, tissues, particle samples and small organisms by means of slicing; various degrees of preservation and visualization
• Study of the organization of intramembrane particles
• Topographic and/or interior nanometric characterization of lipo/lipoprotein compounds, proteins, proteins assemblies, membranes and biomaterials
• Three-dimensional reconstruction of isolated or particle structures
• In situ three-dimensional reconstruction of structures (macromolecules, nanomachines and subcellular bodies) in cell interiors
• Molecular recognition or in situ localization at the subcellular level of proteins, nucleic acids and other macromolecules
• Quantitative studies of measurements or labelling on the nanometric scale
• Basic preparation for transmission optical microscopy

• 3D study of the structure of proteins and macromolecular complexes
• 3D study of cell structures
• Characterization of liposomes, micelles, bicelles and other lipoprotein particles; effect of surfactants; interaction of surfactants with biological material as vehicles for drug delivery
• Study of nanoparticles with peptides, proteins, etc. and their possible entry into tumour cells or through the skin, mucous membranes, haemato-encephalitic barrier, etc.
• Study of functionalized cells or biomolecules on nanostructured substrates
• 3D studies of proteins of pharmacological interest as drug targets
• Characterization of peptide hydrogels
• Ultrastructural study of biological disease models, for example, the malaria parasite infecting the red blood cell or structural changes in tumour cells
• Study of ultrastructural changes in cells caused by drug trials or genetic modifications of different types

Surname	Name	Location	Phone number	Email
DELGADO VALDERRAMA (*)	LIDIA	Edifici Clúster (PCB) C/ Baldiri Reixac, 10	934034860	ldelgado@ccit.ub.edu
MUELA CASTRO	MARIA YOLANDA	Edifici Clúster (PCB) C/ Baldiri Reixac, 10	934034860	yolanda@ccit.ub.edu
PÉREZ PÉREZ	PAULA	Edifici Clúster (PCB) C/ Baldiri Reixac, 10	934034860	paulaperez@ccit.ub.edu

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