Centres Científics i Tecnològics UB

Noticias

10.01.2018

Publicación del artículo “Quantification of interacting cognate odorants with olfactory receptors in nanovesicles”

La Dra. Marta Taulés, técnico de la tecnología de Análisis de Interacciones Moleculares de los CCiTUB, ha participado como coautora en la publicación del artículo: “Quantification of interacting cognate odorants with olfactory receptors in nanovesicles” en la revista Scientific Reports publicada el pasado día 13 de Diciembre.

El resumen del artículo es el siguiente:

"This study aims to improve our understanding of the interaction between olfactory receptors and odorants to develop highly selective biosensing devices. Natural nanovesicles (NVs) from Saccharomyces cerevisiae, ~100nm in diameter, carrying either the human OR17-40 or the chimpanzee OR7D4 olfactory receptor (OR) tagged with the c-myc epitope at their N-terminus, are presented as model systems to quantify the interaction between odorant and olfactory receptors. The level of expression of olfactory receptors was determined at individual NVs using a novel competitive ELISA immunoassay comparing the values obtained against those from techniques involving the solubilization of cell membrane proteins and the identification of c-myc-carrying receptors. Surface Plasmon Resonance (SPR) measurements on L1 Biacore chips indicate that cognate odorants bind to their Ors, thereby quantifying the approximate number of odorants that interact with a given olfactory receptor. The selectivity of OR17-40-carrying NVs towards helional and OR7D4-carrying NVs towards androstenone has been proven in cross-check experiments with non-specific odorant molecules (heptanal and pentadecalactone, respectively) and in control receptors."

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