News10.01.2018Publication of the article "Quantification of interacting cognate odorants with olfactory receptors in nanovesicles" |
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PhD. Marta Taulés, technician of Molecular Interaction Analysis Technology of CCiTUB, has participated as co-author in the publication of the article: "Quantification of interacting cognate odorants with olfactory receptors in nanovesicles" in the journal Scientific Reports published on 13th of December 2017. The summary of the article is as follows: "This study aims to improve our understanding of the interaction between olfactory receptors and odorants to develop highly selective biosensing devices. Natural nanovesicles (NVs) from Saccharomyces cerevisiae, ~100nm in diameter, carrying either the human OR17-40 or the chimpanzee OR7D4 olfactory receptor (OR) tagged with the c-myc epitope at their N-terminus, are presented as model systems to quantify the interaction between odorant and olfactory receptors. The level of expression of olfactory receptors was determined at individual NVs using a novel competitive ELISA immunoassay comparing the values obtained against those from techniques involving the solubilization of cell membrane proteins and the identification of c-myc-carrying receptors. Surface Plasmon Resonance (SPR) measurements on L1 Biacore chips indicate that cognate odorants bind to their Ors, thereby quantifying the approximate number of odorants that interact with a given olfactory receptor. The selectivity of OR17-40-carrying NVs towards helional and OR7D4-carrying NVs towards androstenone has been proven in cross-check experiments with non-specific odorant molecules (heptanal and pentadecalactone, respectively) and in control receptors." For more information, you can access the following link:[+]. |
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