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The Computer Optimized Microscopy Course is directed to researchers (microscopy facility stuff members, pre and post-docs, PIs, etc.) who work with digital images obtained by a range of optical microscopy techniques. The course provides a series of mixed theoretical and practical sessions aimed to show the image processing workflow needed to extract quantitative information over different basic and advanced microscopy techniques. From beginning to end, a first general view of image processing basics will be followed by a more detailed focusing on specific applications.
The open source ImageJ / Fiji software will be used as a main tool for the course. For some specific applications, other open source and commercial softwares will be also utilized, thus providing a wider range of solutions to specific image processing problems.
General Subjects:
Parameters to optimize image acquisition
Introductions to some useful Fiji tools
Image restoration: filters
Image restoration: deconvolution
Segmentation and binnary operations
Automation of the process: Introduction to the macro language
Specific applications:
Colocalization of molecules
Cell tracking
3D reconstruction
FRET acceptor photobleaching and FRAP analysis
Macro programming of some specific applications already seen
High content screening
Machine learning
English.
26th - 30th June, 2017
Fee: 320€. The price includes: registration, one computer for each attendant, the material for the course, the coffee breaks and lunches, and the social dinner.
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Faculty of Biology
University of Barcelona
New building (Prevosti's building) Room 22, Floor -1.
Av. Diagonal 643.
08028 Barcelona
SPAIN
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Hour | Speaker | Topic |
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10:30 - 11:00h | Manel Bosch | Welcome |
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11:00 - 11:30h | | Coffee break |
11:30 - 13:30h | Elena Rebollo | Optimization of image acquisition |
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13:30 - 15:00h | | Lunch |
15:00 - 17:00h | Elena Rebollo | ImageJ / Fiji introduction and tools |
17:00 - 17:30h | | Coffee Break |
17:30 - 19:30h | Anna Bosch | Introduction to macro language |
Hour | Speaker | Topic |
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09:00 - 11:00h | Mª Teresa Castells | Image restoration: filters |
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11:00 - 11:30h | | Coffee break |
11:30 - 13:30h | Sebastien Tosi | Image restoration: Deconvolution |
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13:30 - 15:00h | | Lunch |
15:00 - 17:00h | Manel Bosch | Image segmentation I:
Segmentation methods |
17:00 - 17:30h | | Coffee Break |
17:30 - 19:30h | Anna Bosch | Introduction to macro language |
21:30 | | Social Dinner |
Hour | Speaker | Topic |
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09:00 - 11:00h | Manel Bosch | Image segmentation II:
Binary operations |
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11:00 - 11:30h | | Coffee break |
11:30 - 13:30h | Maria Calvo | Colocalization |
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13:30 - 15:00h | | Lunch |
15:00 - 17:00h | Elena Kardash | FRET analysis |
17:00 - 17:30h | | Coffee Break |
17:30 - 19:30h | Manel Bosch | Macro programming |
Hour | Speaker | Topic |
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09:00 - 11:00h | Elena Rebollo | Cell
tracking |
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11:00 - 11:30h | | Coffee break |
11:30 - 13:30h | Chong Zhang | 3D Reconstruction   |
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13:30 - 15:00h | | Lunch |
15:00 - 17:00h | Diego Megías | FRAP analysis |
17:00 - 17:30h | | Coffee break |
17:30 - 19:30h | Manel Bosch | Macro programming |
Hour | Speaker | Topic |
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09:00 - 11:00h | Diego Megías-Paco Porto | High content screening   |
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11:00 - 11:30h | | Coffee break |
11:30 - 13:30h | Sebastien Tosi | Machine learning |
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13:30 - 15:00h | | Lunch |
15:00 - 17:00h | Manel Bosch | Macro programming |
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Anna Bosch. Centres Científics i Tecnològics. Universitat de Barcelona. CCiTUB
Manel Bosch. Centres Científics i Tecnològics. Universitat de Barcelona. CCiTUB
Maria Calvo. Centres Científics i Tecnològics. Universitat de Barcelona. CCiTUB
Maria Teresa Castells. Servicio de Apoyo a la Investigación. Universidad de Murcia
Elena Kardash. Biochemistry department. University of Geneva
Diego Megías. Centro Nacional de Investigaciones Oncológicas. CNIO
Paco Porto. Leica Microsystems
Elena Rebollo. Institut de Biologia Molecular de Barcelona. IBMB-CSIC
Sebastien Tosi. Institut de Recerca Biomèdica de Barcelona. IRBB
Chong Zhang. SIMBioSys. Universitat Pompeu Fabra.
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WARNING: Registration open until 14th May, 2017. Remember that this is a pre-registration. You will receive a confirmation email during week of May 22th telling if your application is finally accepted or not.
Fee: 320€
(*) Mandatory fields
NOTES: Number of seats is limited. The course is subject to the enrollment of a sufficient number of students. The student is not inscribed into the course until the payment is done. In case of cancelling the enrollment by the student, the student has to inform 15 days before the course starts.You will receive a confirmation email during week of May 22th telling if your application is finally accepted or not.
For more information contact to: admincursos@ccit.ub.edu.
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