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Flow cytometry is able to combine multiple labels on the same sample. Current instruments are capable of detecting a large number of colors simultaneously, but it is essential the design of the experiment to combine fluorochromes properly in order to obtain proper results.
In this seminar the more advanced criteria and strategies in multicolor analysis will be presented for both immunofluorescence (surface and intracellular antigens) and its combination with the detection of fluorescent proteins.
September 29th, 2015 10:30 AM
 Registration
Free access
Location
Aula Félix Serratosa - Edificio Modular PCB
C/ Baldiri Reixac, 10 .
08028 Barcelona
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Program:
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10:30 - 11:15: REVEAL AND ELEVATE: The importance of relative fluorochrome
brightness and antigen density in resolving your population of interest.
(Sergio Navas y Gema Coma. BD Biosciences).
- 11:15 - 12:15: RESOLVE: Managing instument setup and understanding spread to
maximize resolution. Sergio Navas.
- 12:15 - 12:30: Break
- 12:30 - 13:30: ACHIEVE: Putting it all together, practical examples. Gemma Coma.
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Recommendations:
Is advisable to have basic training in RMN (Friebolin and chapters 1-3 of Claridge)
Bibliography:
Basic One-and Two-Dimensional NMR Spectroscopy (second Edition). Horst Friebolin. VCH (1993)
High-Resolution NMR Techniques in Organic Chemistry. Timothy D.W. Claridge. Tetrahedron Organic Chemistry Series Volume 27 (2009)
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Centres Científics i Tecnològics
Universitat de Barcelona
Director: Dr. José Ramón Seoane Trigo
Coordinator:
Dr. Jaume Comas
Technology of Cytometry of CCiTUB.
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