PhD. Manel Bosch, head of the Advanced Optical Microscopy of the CCiTUB located at Diagonal campus, has participated in a study with the aim of evaluating the capacity of an engineered liposomal formulation to fusion with cells and work as drug nanocarriers.
In the study, atomic force microscopy (AFM) has been used in order to observe physicochemical changes on the cell surface provoked by the liposomes contact, and confocal microscopy to determine how they interact with cell membranes, enter the cytoplasm and release their load. The measures with the confocal microscopy have been done with the equipment Zeiss LSM880 located at MOA Diagonal lab of the CCiTUB.
The study has also performed viability assays using methotrexate as an active drug to obtain proof of concept of the formulation’s capacity to function as a drug delivery-system. The results of the different tests performed, corroborate the capacity of the liposomal formulation to serve as a potential nanocarrier.
The work has been published in the journal Colloids and Surfaces B: Biointerfaces, entitled "On the uptake of cationic liposomes by cells: From changes in elasticity to internalization".