Centres Científics i Tecnològics UB

JEM 1010

The JEM 1010 Transmission Electron Microscope (TEM) allows the study of cellular ultrastructure in ultrathin sections (50–70 nm) obtained with an ultramicrotome. Using negative‑staining techniques, it is also possible to study viruses, bacteria, proteins, and amyloid fibres.
This instrument additionally enables the observation of nanoparticle suspensions and their subsequent interaction within tissues.

Equipment Features

• Accelerating voltage – 80 kV
• Tungsten thermionic electron gun
• Sample holder with capacity for two specimens
• Image acquisition system: Gatan Orius CCD camera

Technology Applications

• Ultrastructural, tissue‑level, cellular, and molecular studies
• Analysis of bacterial and viral suspensions, bacteriophages, flagella, etc.
• Ultrastructural studies of isolated cellular organelles, mitochondrial pellets, and chloroplasts
• Whole‑mount studies of cells, bacteria, and similar samples
• Molecular localization and protein colocalization
• Cytochemical studies (enzymatic activities, digestions, etc.)
• Acquisition of bright‑field and dark‑field images

Location

The JEM 1010 TEM is managed by the Electron Microscopy and Related Techniques Unit and is located on the Casanova Campus, at the Faculty of Medicine of the University of Barcelona.